A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Ethanol specifically inhibits NMDA receptors with affinity for ifenprodil in the low micromolar range in cultured cerebellar granule cells
Tekijät: Engblom AC, Courtney MJ, Kukkonen JP, Akerman KEO
Kustantaja: LIPPINCOTT-RAVEN PUBL
Julkaisuvuosi: 1997
Journal: Journal of Neurochemistry
Tietokannassa oleva lehden nimi: JOURNAL OF NEUROCHEMISTRY
Lehden akronyymi: J NEUROCHEM
Vuosikerta: 69
Numero: 5
Aloitussivu: 2162
Lopetussivu: 2168
Sivujen määrä: 7
ISSN: 0022-3042
Tiivistelmä
The effect of ethanol on the intracellular Ca2+ concentration response to NMDA in rat cerebellar granule cells grown in low or high KCI concentrations has been studied using image analysis. The cells grown in low KCI displayed high sensitivity for glycine. The subtype-selective antagonist ifenprodil inhibited the response with high (in the low micromolar range) and low (in the high micromolar range) potency. Ethanol affected the high-potency component in these cultures. In cells grown in high KCI the glycine sensitivity was lower, and a low potency for ifenprodil (high micromolar) dominated. These cells were not significantly sensitive to ethanol. The results indicate that the component displaying potency for ifenprodil in the low micromolar range with properties of the NR2B subunit is the target for ethanol action on the NMDA receptor.
The effect of ethanol on the intracellular Ca2+ concentration response to NMDA in rat cerebellar granule cells grown in low or high KCI concentrations has been studied using image analysis. The cells grown in low KCI displayed high sensitivity for glycine. The subtype-selective antagonist ifenprodil inhibited the response with high (in the low micromolar range) and low (in the high micromolar range) potency. Ethanol affected the high-potency component in these cultures. In cells grown in high KCI the glycine sensitivity was lower, and a low potency for ifenprodil (high micromolar) dominated. These cells were not significantly sensitive to ethanol. The results indicate that the component displaying potency for ifenprodil in the low micromolar range with properties of the NR2B subunit is the target for ethanol action on the NMDA receptor.
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