A3 Vertaisarvioitu kirjan tai muun kokoomateoksen osa

Transposon Insertion Mutagenesis for Archaeal Gene Discovery




TekijätKiljunen Saija, Pajunen Maria, Savilahti Harri

ToimittajaReeves Andrew

Julkaisuvuosi2017

Kokoomateoksen nimiIn Vitro Mutagenesis - Methods and Protocols

Sarjan nimiMethods in Molecular Biology

Numero sarjassa1498

Vuosikerta1498

Aloitussivu309

Lopetussivu320

Sivujen määrä12

ISBN978-1-4939-6470-3

eISBN978-1-4939-6472-7

ISSN1064-3745

DOIhttps://doi.org/10.1007/978-1-4939-6472-7_20


Tiivistelmä

Archaea constitute the third domain of life, but studies on their physiology and other features have lagged behind bacteria and eukarya, largely due to the challenging biology of archaea and concomitant difficulties in methods development. The use of genome-wide en masse insertion mutagenesis is one of the most efficient means to discover the genes behind various biological functions, and such a methodology is described in this chapter for a model archaeon Haloferax volcanii. The strategy successfully employs efficient in vitro transposition in combination with gene targeting in vivo via homologous recombination. The methodology is general and should be transferable to other archaeal species.


Ladattava julkaisu

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Last updated on 2024-26-11 at 22:55