A1 Refereed original research article in a scientific journal

Rapid homogeneous PCR assay for the detection of Chlamydia trachomatis in urine samples




AuthorsLehmusvuori A, Juntunen E, Tapio AH, Rantakokko-Jalava K, Soukka T, Lövgren T

PublisherELSEVIER SCIENCE BV

Publication year2010

JournalJournal of Microbiological Methods

Journal name in sourceJOURNAL OF MICROBIOLOGICAL METHODS

Journal acronymJ MICROBIOL METH

Number in series3

Volume83

Issue3

First page 302

Last page306

Number of pages5

ISSN0167-7012

DOIhttps://doi.org/10.1016/j.mimet.2010.09.018(external)


Abstract
Chlamydia trachomatis infection is the most common bacterial sexually transmitted disease and a major public health problem worldwide. Fast and sensitive point-of-care diagnostics including non-invasive sample collection would be of value for the prevention of C trachomatis transmission. The aim of this study was to develop a fast, reliable, non-invasive and easy-to-use homogenous PCR assay for the detection of C. trachomatis. Bacteria were concentrated from urine by a simple and fast centrifugation-based urine pretreatment method. Novel automated GenomEra technology was utilized for the rapid closed-tube PCR including time-resolved fluorometric detection of the target using lanthanide chelate labeled probes. We have developed a rapid C trachomatis assay which provides qualitative results in 1 h with diagnostic sensitivity and specificity of 98.7% and 97.3%, respectively. The novel assay can be performed with minimal laboratory expertise and without sophisticated DNA-extraction devices and has performance comparable to current gold standard assays. (C) 2010 Elsevier B.V. All rights reserved.



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