A1 Refereed original research article in a scientific journal
Rapid homogeneous PCR assay for the detection of Chlamydia trachomatis in urine samples
Authors: Lehmusvuori A, Juntunen E, Tapio AH, Rantakokko-Jalava K, Soukka T, Lövgren T
Publisher: ELSEVIER SCIENCE BV
Publication year: 2010
Journal: Journal of Microbiological Methods
Journal name in source: JOURNAL OF MICROBIOLOGICAL METHODS
Journal acronym: J MICROBIOL METH
Number in series: 3
Volume: 83
Issue: 3
First page : 302
Last page: 306
Number of pages: 5
ISSN: 0167-7012
DOI: https://doi.org/10.1016/j.mimet.2010.09.018(external)
Abstract
Chlamydia trachomatis infection is the most common bacterial sexually transmitted disease and a major public health problem worldwide. Fast and sensitive point-of-care diagnostics including non-invasive sample collection would be of value for the prevention of C trachomatis transmission. The aim of this study was to develop a fast, reliable, non-invasive and easy-to-use homogenous PCR assay for the detection of C. trachomatis. Bacteria were concentrated from urine by a simple and fast centrifugation-based urine pretreatment method. Novel automated GenomEra technology was utilized for the rapid closed-tube PCR including time-resolved fluorometric detection of the target using lanthanide chelate labeled probes. We have developed a rapid C trachomatis assay which provides qualitative results in 1 h with diagnostic sensitivity and specificity of 98.7% and 97.3%, respectively. The novel assay can be performed with minimal laboratory expertise and without sophisticated DNA-extraction devices and has performance comparable to current gold standard assays. (C) 2010 Elsevier B.V. All rights reserved.
Chlamydia trachomatis infection is the most common bacterial sexually transmitted disease and a major public health problem worldwide. Fast and sensitive point-of-care diagnostics including non-invasive sample collection would be of value for the prevention of C trachomatis transmission. The aim of this study was to develop a fast, reliable, non-invasive and easy-to-use homogenous PCR assay for the detection of C. trachomatis. Bacteria were concentrated from urine by a simple and fast centrifugation-based urine pretreatment method. Novel automated GenomEra technology was utilized for the rapid closed-tube PCR including time-resolved fluorometric detection of the target using lanthanide chelate labeled probes. We have developed a rapid C trachomatis assay which provides qualitative results in 1 h with diagnostic sensitivity and specificity of 98.7% and 97.3%, respectively. The novel assay can be performed with minimal laboratory expertise and without sophisticated DNA-extraction devices and has performance comparable to current gold standard assays. (C) 2010 Elsevier B.V. All rights reserved.
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