A1 Refereed original research article in a scientific journal
Crystallization and preliminary crystallographic data of SnoaL, a polyketide cyclase in nogalamycin biosynthesis
Authors: Sultana A, Kallio P, Jansson A, Niemi J, Mantsala P, Schneider G
Publisher: BLACKWELL MUNKSGAARD
Publication year: 2004
Journal:: Acta Crystallographica Section D: Biological Crystallography
Journal name in source: ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY
Journal acronym: ACTA CRYSTALLOGR D
Volume: 60
First page : 1118
Last page: 1120
Number of pages: 3
ISSN: 0907-4449
DOI: https://doi.org/10.1107/S090744490400705X
Abstract
Nogalonic acid methyl ester cyclase (SnoaL) catalyzes the last ring-closure step in the biosynthesis of the polyketide antibiotic nogalamycin. Crystals of a complex of SnoaL with the substrate nogalonic acid methyl ester have been obtained using PEG 4000 as precipitant. The crystals are orthorhombic, space group I222, with unit-cell parameters a = 69.1, b = 72.0, c = 65.4 Angstrom. They diffract to 1.35 Angstrom resolution using synchrotron radiation. A Matthews coefficient of 2.0 Angstrom(3) Da(-1) suggests one subunit in the asymmetric unit. Diffraction data for an isomorphous uranium derivative were collected and a difference Patterson map showed strong peaks which allowed determination of the position of the uranium ions.
Nogalonic acid methyl ester cyclase (SnoaL) catalyzes the last ring-closure step in the biosynthesis of the polyketide antibiotic nogalamycin. Crystals of a complex of SnoaL with the substrate nogalonic acid methyl ester have been obtained using PEG 4000 as precipitant. The crystals are orthorhombic, space group I222, with unit-cell parameters a = 69.1, b = 72.0, c = 65.4 Angstrom. They diffract to 1.35 Angstrom resolution using synchrotron radiation. A Matthews coefficient of 2.0 Angstrom(3) Da(-1) suggests one subunit in the asymmetric unit. Diffraction data for an isomorphous uranium derivative were collected and a difference Patterson map showed strong peaks which allowed determination of the position of the uranium ions.
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