A1 Refereed original research article in a scientific journal

Human rhinovirus in experimental infection after peroral Lactobacillus rhamnosus GG consumption, a pilot study




AuthorsTapiovaara L, Kumpu M, Makivuokko H, Waris M, Korpela R, Pitkaranta A, Winther B

PublisherWILEY-BLACKWELL

Publication year2016

JournalInternational Forum of Allergy and Rhinology

Journal name in sourceINTERNATIONAL FORUM OF ALLERGY & RHINOLOGY

Journal acronymINT FORUM ALLERGY RH

Volume6

Issue8

First page 848

Last page853

Number of pages6

ISSN2042-6976

eISSN2042-6984

DOIhttps://doi.org/10.1002/alr.21748


Abstract
Background: Data has emerged on possible beneficial effects of probiotics in respiratory tract viral infections, but it is unclear if the promising positive effects evidenced are due to a reduced viral load during infections. The aims of this work were to investigate the effect of peroral probiotic Lactobacillus rhamnosus GG (American Type Culture Collection [ATCC], Accession No. 53103) consumption on human rhinovirus (HRV) load in nasopharyngeal lavage samples in experimental HRV infection, and to correlate viral load to clinical symptoms.Methods: Intranasal HRV A39 inoculation was performed on 59 adults, who had consumed juice enriched with live or heat-inactivated L. rhamnosus GG or control juice for 3 weeks prior to inoculation in a randomized, controlled, pilot trial setting. Nasopharyngeal lavage samples and symptom data were analyzed on day 0 before inoculation, and on days 2 and 5. Samples were subjected to quantitative HRV detection by polymerase chain reaction (PCR).Results: Before inoculation 9 of 59 (15%) samples presented with another HRV strain than the studied A39. There was a tendency toward the lowest HRV loads in the L. rhamnosus GG groups and the highest in placebo group (log(10) copies/mL, 95% confidence interval [CI], 6.20 [5.18 to 7.40] in live, 6.30 [4.91 to 7.08] in inactivated L. rhamnosus GG, and 7.25 [5.81 to 7.52] in placebo group, p = 0.57 in day 2) in the wild-type excluded population. The HRV load positively correlated with the symptom scores on days 2 and 5 (correlation coefficient 0.61 [p < 0.001] and 0.28 [p = 0.034], respectively).Conclusion: Results did not show statistical differences in viral loads in subjects using L. rhamnosus GG when compared to placebo. HRV load positively correlated with the total symptom scores. (C) 2016 ARS-AAOA, LLC.



Last updated on 2024-26-11 at 18:53