A1 Refereed original research article in a scientific journal

IFN-α/IFN-λ responses to respiratory viruses in paediatric asthma




AuthorsBergauer A, Sopel N, Kroß B, Vuorinen T, Xepapadaki P, Weiss ST, Blau A, Sharma H, Kraus C, Springel R, Rauh M, Mittler S, Graser A, Zimmermann T, Melichar VO, Kiefer A, Kowalski ML, Sobanska A, Jartti T, Lukkarinen H, Papadopoulos NG, Finotto S.

PublisherEUROPEAN RESPIRATORY SOC JOURNALS LTD

Publication year2017

JournalEuropean Respiratory Journal

Journal name in sourceEUROPEAN RESPIRATORY JOURNAL

Journal acronymEUR RESPIR J

Article number1600969

Volume49

Issue2

Number of pages10

ISSN0903-1936

eISSN1399-3003

DOIhttps://doi.org/10.1183/13993003.00969-2016


Abstract

We analysed the influence of rhinovirus (RV) in nasopharyngeal fluid (NPF) on type I and III interferon (IFN) responses (e.g. IFN-α and IFN-λ) and their signal transduction, at baseline and during disease exacerbation, in cohorts of pre-school children with and without asthma.

At the time of recruitment into the Europe-wide study PreDicta, and during symptoms, NPF was collected and the local RV colonisation was analysed. Peripheral blood mononuclear cells (PBMCs) were challenged in vitro with RV or not. RNA was analysed by quantitative real-time PCR and gene arrays. Serum was analysed with ELISA for IFNs and C-reactive protein.

We found that PBMCs from asthmatic children infected in vitro with the RV1b serotype upregulated MYD88IRF1STAT1 and STAT2 mRNA, whereas MYD88IRF1STAT1 and IRF9 were predominantly induced in control children. Moreover, during symptomatic visits because of disease exacerbation associated with RV detection in NPF, IFN-α production was found increased, while IFN-λ secretion was already induced by RV in asthmatic children at baseline.

During asthma exacerbations associated with RV, asthmatic children can induce IFN-α secretion, indicating a hyperactive immune response to repeated respiratory virus infection.



Last updated on 2024-26-11 at 21:17