SIMULTANEOUS ESTIMATION OF TRAMADOL HYDROCHLORIDE, PARACETAMOL AND DOMPERIDONE BY RP-HPLC IN TABLET FORMULATION

: Jain D, Kachave RN, Bhadane RN

Publisher: TAYLOR & FRANCIS INC

: 2010

Journal of Liquid Chromatography and Related Technologies

: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES

: J LIQ CHROMATOGR R T

: PII 920292159

: 33

: 786

: 792

: 7

: 1082-6076

DOI: https://doi.org/10.1080/10826071003684224

A new high performance liquid chromatography method was developed and validated for the quantitation of tramadol, paracetamol, and domperidone in pharmaceutical formulations. Determination was performed using a Inertsil-ODS-3 (C-18) column (5m, 250mmx4.60mm), a mobile phase containing methanol-phosphate buffer (pH 4.0; 40+60, v/v), in gradient flow rate 1.2mL. The method was validated with respect to linearity, precision, robustness, and accuracy. The calibration graphs ranged from 250 to 1500mg/mL for paracetamol, 25 to 150mg/mL for tramadol, and 5 to 30mg/mL for domperidone. Intra- and interday relative standard deviation values for the standard solutions were 0.077%, 0.98%, and 1.04%, respectively. Repeatability of relative standard deviation values was 0.115%, 0.494%, and 1.97% respectively. Total recoveries of paracetamol, tramadol, and domperidone from the laboratory prepared mixtures were 100.4, 100.06, and 100.2%, respectively.