1H NMR-based metabolic fingerprinting of urine metabolites after consumption of lingonberries (Vaccinium vitis-idaea) with a high-fat meal

: Lehtonen HM, Lindstedt A, Järvinen R, Sinkkonen J, Graça G, Viitanen M, Kallio H, Gil AM

: Elsevier

Publisher: ELSEVIER SCI LTD

: 2013

: Food Chemistry

: FOOD CHEMISTRY

: FOOD CHEM

: 2-3

: 138

: 2-3

: 982

: 990

: 9

: 0308-8146

DOI: https://doi.org/10.1016/j.foodchem.2012.10.081

The use of NMR metabolomics in clinical trials is growing; however, reports of postprandial experiments in humans are scarce. The present study investigated whether consumption of lingonberries as a supplement to an oil-rich meal modifies the postprandial fingerprints of human urine. Urine samples were analysed by H-1 NMR, and untargeted multivariate analysis was applied to the data for comprehensive fingerprinting. A clear separation of postprandial lingonberry meal samples was revealed. To evaluate statistical differences, a targeted approach was applied for the informative spectral areas. Significantly (p < 0.05) increased levels of polyphenol metabolites, hippuric acid and 4-hydroxyhippuric acid, and decreased creatinine and dimethylamine levels were the major explanations for the grouping of the postprandial samples after the different meals. Thus, inclusion of polyphenol-rich lingonberry powder in a rapeseed oil-rich meal modifies the metabolic profile of urine which may be used to reveal both consumption of berries and health-promoting changes in the common metabolism. (C) 2012 Elsevier Ltd. All rights reserved.