Background: Prospective data on the impact of CD34⁺ cell loss during cryopreservation and the amount of cryopreserved CD34⁺ cells infused after high-dose therapy on hematologic recovery and post-transplant outcome in multiple myeloma (MM) are scarce.

Patients and methods: This post-hoc study aimed to investigate factors associating with CD34⁺ cell loss during cryopreservation and the effects of the infusion of a very low number (<1.0 × 10⁶ /kg, group A), low number (1-1.9 × 10⁶ /kg, group B), and optimal number (≥2 × 10⁶ /kg, group C) of thawed viable CD34⁺ cells on hematologic recovery, progression free survival, and overall survival after autologous stem cell transplantation among 127 patients with MM.

Results: In group C, pegfilgrastim use (P = 0.001), plerixafor use (P = 0.039), and older age ≥ 60 years (P = 0.026) were associated with less loss of CD34⁺ cells during cryopreservation. Better mobilization efficacy correlated with greater CD34⁺ cell loss in group B (P = 0.013 and P = 0.001) and in group C (P < 0.001 and P < 0.001). Early platelet engraftment was slowest in group A (20 d vs 12 d in group B vs 11 d in group C, P = 0.003). The infused viable CD34⁺ cell count <1.0 × 10⁶ /kg seemed not to have influence on PFS (P = 0.322) or OS (P = 0.378) in MM patients.

Conclusions: Cryopreservation impacts significantly on the CD34⁺ cell loss. A very low number of graft viable CD34⁺ cells did not affect PFS or OS.