Refereed journal article or data article (A1)

Determination of Bioactive Compounds, Antioxidant Capacities and Safety of the Somatic Hybrid Potatoes




List of Authors: Kammoun Mariem, Jeddou Khaoula Ben, Rokka Veli-Matti, Pihlava Juha-Matti, Hellström Jarkko, Gutierrez-Quequezana Liz, Essid Mohammed Farouk, Gargouri-Bouzid Radhia, Nouri-Ellouz Oumèma

Publisher: SPRINGER

Publication year: 2022

Journal: Potato Research

Journal name in source: POTATO RESEARCH

Journal acronym: POTATO RES

Number of pages: 22

ISSN: 0014-3065

eISSN: 1871-4528

DOI: http://dx.doi.org/10.1007/s11540-022-09559-8

URL: https://link.springer.com/article/10.1007/s11540-022-09559-8


Abstract

Intraspecific somatic hybrids (CN1 and CN2) produced by protoplast fusion between dihaploid lines of potato cultivars Cardinal and Nicola were analysed in terms of their bioactive compounds and antioxidant activities. The total phenolic compound levels were investigated from the extracts derived from whole tubers using the Folin-Ciocalteu ethanol reagent method, which demonstrated that CN1 and CN2 hybrids contained higher levels of phenolics than the tubers of cv. Nicola. The corresponding extracts were also used for determination of the bioactivity levels, and an increased antioxidant capacity in the extracts of hybrid potato was revealed by DPPH and ABTS radical-scavenging tests compared to the tuber extracts of cv. Nicola. The identification of bioactive compounds in tubers was carried out separately for peel and flesh compartments to determine the phenolic compounds: phenolic acids by UHPLC-DAD and anthocyanins by HPLC-DAD and LC-MS. Glycoalkaloids were determined by UPLC-QTOF MS analysis. Phenolic acid profiling showed the presence of caffeic acid and three caffeoylquinic acid isomeric forms (3-CQA, 4-CQA and 5-CQA) in the peels, whereas only caffeic acid and 5-CQA (chlorogenic acid) were detected in the flesh compartments. The phenolic acid profiles of the somatic hybrids did not remarkably differ from the corresponding profiles of cv. Nicola. The colour-pigmented peels of the somatic hybrids were also analysed for their anthocyanin content, and six substances were identified, four of them were acylated glucosides of pelargonidin and peonidin by coumaric or ferulic acids. The increased antioxidative activities determined in the somatic hybrid tubers are most probably linked to their higher phenolic compound levels due to the pigmented tuber skin colour. The somatic hybrids had alpha-solanine and alpha-chaconine as the major glycoalkaloid compounds, and their total quantities were less than that found in tubers of cv. Nicola. This confirms the safety of the hybrid tubers for human consumption.


Last updated on 2022-16-08 at 13:26