Refereed article in compilation book (A3)

Functional Genomics of Fish Erythrocytes




List of Authors: Nikinmaa Mikko, Crespel Amélie

Edition name or number: 1

Publisher: Humana Press Inc.

Publication year: 2022

Book title *: Marine Genomics: Methods and Protocols

Journal name in source: Methods in Molecular Biology

Title of series: Methods in Molecular Biology

Volume number: 2498

ISBN: 978-1-0716-2312-1

eISBN: 978-1-0716-2313-8

ISSN: 1064-3745

DOI: http://dx.doi.org/10.1007/978-1-0716-2313-8_22

URL: https://link.springer.com/protocol/10.1007/978-1-0716-2313-8_22


Abstract

Fish red blood cells contain nucleus and therefore carry on active transcription throughout their life. As the steady state level of mRNA depends both on the production and breakdown of the mRNA, it is important to pay attention to sampling, cell separation, mRNA extraction, and transcript stability to conduct a comprehensive erythrocyte genomics study. In addition, particular attention should be made to tie the transcriptional changes to corresponding protein activities, as only those would impact cellular functions. Here, we thus describe the different steps to conduct comprehensive functional genomics studies with fish erythrocytes.


Last updated on 2022-16-08 at 09:57