Refereed journal article or data article (A1)

Interactions between Hydrolysable Tannins and Lipid Vesicles from Escherichia coli with Isothermal Titration Calorimetry




List of Authors: Virtanen Valtteri, Green Rebecca J, Karonen Maarit

Publisher: MDPI

Publication year: 2022

Journal: Molecules

Journal name in source: MOLECULES

Journal acronym: MOLECULES

Volume number: 27

Issue number: 10

Number of pages: 18

eISSN: 1420-3049

DOI: http://dx.doi.org/10.3390/molecules27103204

URL: https://www.mdpi.com/1420-3049/27/10/3204

Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/175887739


Abstract

Isothermal titration calorimetry (ITC) was used to study the interactions between hydrolysable tannins (HTs) and lipid vesicles prepared from a phospholipid extract of Escherichia coli (E. coli). A group of 24 structurally different HTs was selected, and structural differences affecting their affinities to interact with lipid vesicles in aqueous buffered media were identified. In general, the interactions between HTs and lipid vesicles were exothermic in nature, and ITC as a technique functioned well in the screening of HTs for their affinity for lipids. Most notably, the galloyl moiety, the structural flexibility of the entire tannin structure, the hydrophobicity of the tannin, and higher molecular weight were observed to be important for the stronger interactions with the lipids. The strongest interactions with lipids were observed for rugosins D and G. It was also observed that some HTs with moderate hydrophobicities, such as geraniin, chebulagic acid, and chebulinic acid, did not have any detectable interactions with the lipid vesicles, suggesting that a hydrophobic structure alone does not guarantee an affinity for lipids.


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Last updated on 2022-10-08 at 13:48