Vertaisarvioitu alkuperäisartikkeli tai data-artikkeli tieteellisessä aikakauslehdessä (A1)
Interactions between Hydrolysable Tannins and Lipid Vesicles from Escherichia coli with Isothermal Titration Calorimetry
Julkaisun tekijät: Virtanen Valtteri, Green Rebecca J, Karonen Maarit
Kustantaja: MDPI
Julkaisuvuosi: 2022
Journal: Molecules
Tietokannassa oleva lehden nimi: MOLECULES
Lehden akronyymi: MOLECULES
Artikkelin numero: 3204
Volyymi: 27
Julkaisunumero: 10
Sivujen määrä: 18
eISSN: 1420-3049
DOI: http://dx.doi.org/10.3390/molecules27103204
Verkko-osoite: https://www.mdpi.com/1420-3049/27/10/3204
Rinnakkaistallenteen osoite: https://research.utu.fi/converis/portal/detail/Publication/175887739
Isothermal titration calorimetry (ITC) was used to study the interactions between hydrolysable tannins (HTs) and lipid vesicles prepared from a phospholipid extract of Escherichia coli (E. coli). A group of 24 structurally different HTs was selected, and structural differences affecting their affinities to interact with lipid vesicles in aqueous buffered media were identified. In general, the interactions between HTs and lipid vesicles were exothermic in nature, and ITC as a technique functioned well in the screening of HTs for their affinity for lipids. Most notably, the galloyl moiety, the structural flexibility of the entire tannin structure, the hydrophobicity of the tannin, and higher molecular weight were observed to be important for the stronger interactions with the lipids. The strongest interactions with lipids were observed for rugosins D and G. It was also observed that some HTs with moderate hydrophobicities, such as geraniin, chebulagic acid, and chebulinic acid, did not have any detectable interactions with the lipid vesicles, suggesting that a hydrophobic structure alone does not guarantee an affinity for lipids.
Ladattava julkaisu This is an electronic reprint of the original article. |