A1 Refereed original research article in a scientific journal

Cardiomyocyte Apoptosis after Cardioplegic Ischemia: Comparison to Unprotected Regional Ischemia-Reperfusion




AuthorsMalmberg M, Parkka J, Vahasilta T, Saraste A, Laitio T, Kiss J, Latva-Hirvela J, Saukko P, Savunen T

PublisherKARGER

Publication year2011

JournalEuropean Surgical Research

Journal name in sourceEUROPEAN SURGICAL RESEARCH

Journal acronymEUR SURG RES

Number in series1

Volume46

Issue1

First page 19

Last page25

Number of pages7

ISSN0014-312X

DOIhttps://doi.org/10.1159/000321875(external)


Abstract
Background: Cardiomyocyte apoptosis might contribute to left ventricular (LV) dysfunction following cardiac surgery. Magnetic resonance imaging is considered the most accurate method of determining LV function. We compared apoptosis (by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling, TUNEL, staining and detection of caspase 3 activation) and LV function after regional ischemia-reperfusion (I-R) and global cardioplegic ischemia. Methods: Pigs were randomized to undergo regional myocardial I-R for 20 + 20 min, global myocardial ischemia with cardiopulmonary bypass (CPB) for 40 min or CPB without ischemia (control), followed by 274 min of reperfusion. Results: Compared with the control group, the number of TUNEL-positive cardiomyocytes was higher in the global ischemia group with CPB (0.024 +/- 0.014%; p = 0.02) and further increased in areas of unprotected regional I-R (0.444 +/- 0.562%; p = 0.003, vs. control). Myocytes with active caspase 3 were detected after global and regional ischemia. The global ejection fraction did not differ between CPB and regional I-R groups. Conclusions: The use of cardioplegia and CPB efficiently protects the heart from global I-R-induced cardiomyocyte apoptosis during open heart surgery. Copyright (C) 2010 S. Karger AG, Basel



Last updated on 2024-26-11 at 14:25