Refereed journal article or data article (A1)

Structure and enzymatic mechanism of a moonlighting dUTPase




List of Authors: Leveles I, Nemeth V, Szabo JE, Harmat V, Nyiri K, Bendes AA, Papp-Kadar V, Zagyva I, Rona G, Ozohanics O, Vekey K, Toth J, Vertessy BG

Publisher: INT UNION CRYSTALLOGRAPHY

Publication year: 2013

Journal: Acta crystallographica section D : structural biology

Journal name in source: ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY

Journal acronym: ACTA CRYSTALLOGR D

Volume number: 69

Number of pages: 11

ISSN: 2059-7983

DOI: http://dx.doi.org/10.1107/S0907444913021136


Abstract
Genome integrity requires well controlled cellular pools of nucleotides. dUTPases are responsible for regulating cellular dUTP levels and providing dUMP for dTTP biosynthesis. In Staphylococcus, phage dUTPases are also suggested to be involved in a moonlighting function regulating the expression of pathogenicity-island genes. Staphylococcal phage trimeric dUTPase sequences include a specific insertion that is not found in other organisms. Here, a 2.1 angstrom resolution three-dimensional structure of a phi 11 phage dUTPase trimer with complete localization of the phage-specific insert, which folds into a small beta-pleated mini-domain reaching out from the dUTPase core surface, is presented. The insert mini-domains jointly coordinate a single Mg2+ ion per trimer at the entrance to the threefold inner channel. Structural results provide an explanation for the role of Asp95, which is suggested to have functional significance in the moonlighting activity, as the metal-ion-coordinating moiety potentially involved in correct positioning of the insert. Enzyme-kinetics studies of wild-type and mutant constructs show that the insert has no major role in dUTP binding or cleavage and provide a description of the elementary steps (fast binding of substrate and release of products). In conclusion, the structural and kinetic data allow insights into both the phage-specific characteristics and the generally conserved traits of phi 11 phage dUTPase.


Last updated on 2022-27-09 at 08:16