Refereed journal article or data article (A1)

Single-step noncompetitive immunocomplex immunoassay for rapid aflatoxin detection




List of Authors: Peltomaa Riikka, Abbas Asmaa, Yli-Mattila Tapani, Lamminmäki Urpo

Publisher: Elsevier Ltd

Publication year: 2022

Journal: Food Chemistry

Journal name in source: Food Chemistry

Volume number: 392

eISSN: 1873-7072

DOI: http://dx.doi.org/10.1016/j.foodchem.2022.133287

URL: https://doi.org/10.1016/j.foodchem.2022.133287

Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/175544789


Abstract

Owing to the high carcinogenicity of aflatoxins, these toxic secondary metabolites pose a severe risk to human and animal health and can have major economic implications. Herein, we report the development of a noncompetitive immunoassay for aflatoxins based on a monoclonal capture antibody and a unique anti-immunocomplex (anti-IC) antibody fragment (scFv) isolated from a synthetic antibody repertoire. The anti-IC scFv recognizes the immunocomplex and enables the development of noncompetitive sandwich-type assays despite the small size of the analyte. The single-step assay developed in this work, with a detection limit of 70 pg mL−1, could detect aflatoxins within 15 min. The assay was applied to the analysis of spiked food samples, and the results showed that the method could provide a rapid and simple tool for aflatoxin detection. Moreover, the work demonstrates the potential of anti-IC antibodies and non-competitive immunoassays for the analysis of small molecule contaminants.


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Last updated on 2022-28-09 at 12:57