Refereed journal article or data article (A1)

Mussel-Inspired and Bioclickable Peptide Engineered Surface to Combat Thrombosis and Infection




List of Authors: Mou Xiaohui, Zhang Hongbo, Qiu Hua, Zhang Wentai, Wang Ying, Xiong Kaiqin, Huang Nan, Santos Hélder A., Yang Zhilu

Publisher: AMER ASSOC ADVANCEMENT SCIENCE

Publication year: 2022

Journal: Research

Journal name in source: RESEARCH

Journal acronym: RESEARCH-CHINA

Volume number: 2022

Number of pages: 14

ISSN: 2096-5168

DOI: http://dx.doi.org/10.34133/2022/9780879

URL: https://spj.sciencemag.org/journals/research/2022/9780879/

Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/175238675


Abstract
Thrombosis and infections are the two major complications associated with extracorporeal circuits and indwelling medical devices, leading to significant mortality in clinic. To address this issue, here, we report a biomimetic surface engineering strategy by the integration of mussel-inspired adhesive peptide, with bio-orthogonal click chemistry, to tailor the surface functionalities of tubing and catheters. Inspired by mussel adhesive foot protein, a bioclickable peptide mimic (DOPA)(4)-azide-based structure is designed and grafted on an aminated tubing robustly based on catechol-amine chemistry. Then, the dibenzylcyclooctyne (DBCO) modified nitric oxide generating species of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) chelated copper ions and the DBCO-modified antimicrobial peptide (DBCO-AMP) are clicked onto the grafted surfaces via bio-orthogonal reaction. The combination of the robustly grafted AMP and Cu-DOTA endows the modified tubing with durable antimicrobial properties and ability in long-term catalytically generating NO from endogenous s-nitrosothiols to resist adhesion/activation of platelets, thus preventing the formation of thrombosis. Overall, this biomimetic surface engineering technology provides a promising solution for multicomponent surface functionalization and the surface bioengineering of biomedical devices with enhanced clinical performance.

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Last updated on 2022-20-05 at 13:07