Refereed journal article or data article (A1)
Mussel-Inspired and Bioclickable Peptide Engineered Surface to Combat Thrombosis and Infection
List of Authors: Mou Xiaohui, Zhang Hongbo, Qiu Hua, Zhang Wentai, Wang Ying, Xiong Kaiqin, Huang Nan, Santos Hélder A., Yang Zhilu
Publisher: AMER ASSOC ADVANCEMENT SCIENCE
Publication year: 2022
Journal: Research
Journal name in source: RESEARCH
Journal acronym: RESEARCH-CHINA
Article number: 9780879
Volume number: 2022
Number of pages: 14
ISSN: 2096-5168
DOI: http://dx.doi.org/10.34133/2022/9780879
URL: https://spj.sciencemag.org/journals/research/2022/9780879/
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/175238675
Thrombosis and infections are the two major complications associated with extracorporeal circuits and indwelling medical devices, leading to significant mortality in clinic. To address this issue, here, we report a biomimetic surface engineering strategy by the integration of mussel-inspired adhesive peptide, with bio-orthogonal click chemistry, to tailor the surface functionalities of tubing and catheters. Inspired by mussel adhesive foot protein, a bioclickable peptide mimic (DOPA)(4)-azide-based structure is designed and grafted on an aminated tubing robustly based on catechol-amine chemistry. Then, the dibenzylcyclooctyne (DBCO) modified nitric oxide generating species of 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) chelated copper ions and the DBCO-modified antimicrobial peptide (DBCO-AMP) are clicked onto the grafted surfaces via bio-orthogonal reaction. The combination of the robustly grafted AMP and Cu-DOTA endows the modified tubing with durable antimicrobial properties and ability in long-term catalytically generating NO from endogenous s-nitrosothiols to resist adhesion/activation of platelets, thus preventing the formation of thrombosis. Overall, this biomimetic surface engineering technology provides a promising solution for multicomponent surface functionalization and the surface bioengineering of biomedical devices with enhanced clinical performance.
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