Multiplex PCR is
a powerful method to detect, identify and quantify the mycotoxigenic fungus by
targeting the amplification of genes associated with mycotoxin production and
detection, identification and quantification of Fusarium species. As compared with uniplex PCR, it has several
advantages such as low cost, shortened time, simultaneous amplification of more
than two genes (in only one reaction tube) etc. Here, we describe multiplex
PCR-based detection and identification of trichothecene-, zearalenone, fumonisin
and enniatin-producing Fusarium species,
the use of multiplex PCR in multiplex genotype assay and the use of multiplex
TaqMan real-time qPCR.