A1 Refereed original research article in a scientific journal
Quality of Protein Isolates and Hydrolysates from Baltic Herring (Clupea harengus membras) and Roach (Rutilus rutilus) Produced by pH-Shift Processes and Enzymatic Hydrolysis
Authors: Kakko Tanja, Damerau Annelie, Nisov Anni, Puganen Anna, Tuomasjukka Saska, Honkapää Kaisu, Tarvainen Marko, Yang Baoru
Publisher: MDPI
Publication year: 2022
Journal: Foods
Journal name in source: FOODS
Journal acronym: FOODS
Article number: 230
Volume: 11
Issue: 2
Number of pages: 22
DOI: https://doi.org/10.3390/foods11020230
Web address : https://www.mdpi.com/2304-8158/11/2/230
Self-archived copy’s web address: https://research.utu.fi/converis/portal/detail/Publication/174815490
Fractionation is a potential way to valorize under-utilized fishes, but the quality of the resulting fractions is crucial in terms of their applicability. The aim of this work was to study the quality of protein isolates and hydrolysates extracted from roach (Rutilus rutilus) and Baltic herring (Clupea harengus membras) using either pH shift or enzymatic hydrolysis. The amino acid composition of protein isolates and hydrolysates mostly complied with the nutritional requirements for adults, but protein isolates produced using pH shift showed higher essential to non-essential amino acid ratios compared with enzymatically produced hydrolysates, 0.84-0.85 vs. 0.65-0.70, respectively. Enzymatically produced protein hydrolysates had a lower total lipid content, lower proportion of phospholipids, and exhibited lower degrees of protein and lipid oxidation compared with pH-shift-produced isolates. These findings suggest enzymatic hydrolysis to be more promising from a lipid oxidation perspective while the pH-shift method ranked higher from a nutrient perspective. However, due to the different applications of protein isolates and hydrolysates produced using pH shift or enzymatic hydrolysis, respectively, the further optimization of both studied methods is recommended.
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