A1 Refereed original research article in a scientific journal
Predominance of oleanane-type ginsenoside R-0 and malonyl esters of protopanaxadiol-type ginsenosides in the 20-year-old suspension cell culture of Panax japonicus CA Meyer
Authors: Glagoleva Elena S., Konstantinova Svetlana V., Kochkin Dmitry V., Ossipov Vladimir, Titova Maria V., Popova Elena V., Nosov Alexander M., Paek Kee-Yoeup
Publisher: ELSEVIER
Publication year: 2022
Journal: Industrial Crops and Products
Journal name in source: INDUSTRIAL CROPS AND PRODUCTS
Journal acronym: IND CROP PROD
Article number: 114417
Volume: 177
Number of pages: 9
ISSN: 0926-6690
DOI: https://doi.org/10.1016/j.indcrop.2021.114417
Abstract
Cell culture of Panax spp. is a potent sustainable source of biologically active triterpene glycosides (ginsenosides) specific for this genus. In this study, growth and biosynthetic profiles were investigated for P. japonicus suspension cell culture maintained for over 20 years by periodic subcultures in flasks. Cell culture demonstrated intensive growth evidenced by high accumulation of dry weight (DW, 9.8 gL(-1)), viability (80-90%) and specific growth rate (0.12 day(-1)) that were comparable to those recorded at the year of culture induction. Nineteen structurally different ginsenosides were identified in cell biomass using UPLC-Q-Exactive-Orbitrap HRMS and HPLC-ESI-Q-MS. Ginsenoside Ro and malonyl-ginsenosides (MalGs) accounted for over 80% of the total ginsenoside content in cells during the subculture cycle while the sum of neutral ginsenosides of protopanaxatriol (PPT: Re, Rg(1), Rf) and protopanaxadiol (PPD: Rb-1, Rb-2, Re, Rd) groups constituted less than 20%. Ginsenoside contents were maximized during growth decline phase as 35.3 mg/gDW for R-0, 48.7 mg/gDW for MalGs, 10.4 mg/gDW for PPD and 5.2 mg/gDW for PPT. These data confirmed that 20-year-old cell culture of P. japonicus retained the ability for intensive growth and accumulation of wide spectrum of ginsenosides which opens the door for wide application of this culture.
Cell culture of Panax spp. is a potent sustainable source of biologically active triterpene glycosides (ginsenosides) specific for this genus. In this study, growth and biosynthetic profiles were investigated for P. japonicus suspension cell culture maintained for over 20 years by periodic subcultures in flasks. Cell culture demonstrated intensive growth evidenced by high accumulation of dry weight (DW, 9.8 gL(-1)), viability (80-90%) and specific growth rate (0.12 day(-1)) that were comparable to those recorded at the year of culture induction. Nineteen structurally different ginsenosides were identified in cell biomass using UPLC-Q-Exactive-Orbitrap HRMS and HPLC-ESI-Q-MS. Ginsenoside Ro and malonyl-ginsenosides (MalGs) accounted for over 80% of the total ginsenoside content in cells during the subculture cycle while the sum of neutral ginsenosides of protopanaxatriol (PPT: Re, Rg(1), Rf) and protopanaxadiol (PPD: Rb-1, Rb-2, Re, Rd) groups constituted less than 20%. Ginsenoside contents were maximized during growth decline phase as 35.3 mg/gDW for R-0, 48.7 mg/gDW for MalGs, 10.4 mg/gDW for PPD and 5.2 mg/gDW for PPT. These data confirmed that 20-year-old cell culture of P. japonicus retained the ability for intensive growth and accumulation of wide spectrum of ginsenosides which opens the door for wide application of this culture.
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