A1 Journal article – refereed

Cleavage of short oligoribonucleotides by a Zn2+ binding multi-nucleating azacrown conjugate




List of Authors: Maarit Laine, Tuomas Lönnberg, Mia Helkearo, Harri Lönnberg

Publisher: ELSEVIER SCIENCE SA

Publication year: 2016

Journal: Inorganica Chimica Acta

Volume number: 452

Issue number: SI

Number of pages: 7

ISSN: 0020-1693

DOI: http://dx.doi.org/10.1016/j.ica.2015.12.030


Abstract

A multi-nucleating azacrown conjugate (5a) consisting of two 3,5-bis(1,5,9-triazacyclododecan-3-yloxy-methyl)benzyl groups attached to 1 and 7 sites of cyclen was prepared and tested as an artificial ribonuclease. The conjugate in the presence of five equivalents of zinc nitrate expectedly showed uridine selectivity comparable to that 1,3,5-tris(1,5,9-triazacyclododecan-3-yl)benzene (2), a compound known to bind to two adjacent uridine residues and cleave the intervening phosphodiester bond. 5a was, however, unable to discriminate between two and three adjacent uridine residues, but cleaved oligonucleotides containing a UpU and UpUpU site at a comparable rate, even when present at sub-saturating concentrations. (C) 2016 Elsevier B.V. All rights reserved.


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