A1 Refereed original research article in a scientific journal
Laboratory course on Streptomyces genetics and secondary metabolism
Authors: Siitonen V, Raty K, Metsa-Ketela M
Publisher: John Wiley & Sons, Inc.
Publication year: 2016
Journal: Biochemistry and Molecular Biology Education
Volume: 44
Issue: 5
First page : 492
Last page: 499
Number of pages: 8
ISSN: 1470-8175
eISSN: 1539-3429
DOI: https://doi.org/10.1002/bmb.20970(external)
Abstract
The "Streptomyces genetics and secondary metabolism" laboratory course gives an introduction to the versatile soil dwelling Gram-positive bacteria Streptomyces and their secondary metabolism. The course combines genetic modification of Streptomyces; growing of the strain and protoplast preparation, plasmid isolation by alkaline lysis and phenol precipitation, digestions, and ligations prior to protoplast transformation, as well as investigating the secondary metabolites produced by the strains. Thus, the course is a combination of microbiology, molecular biology, and chemistry. After the course the students should understand the relationship between genes, proteins, and the produced metabolites. 2016 by The International Union of Biochemistry and Molecular Biology, 44(5):492-499, 2016.
The "Streptomyces genetics and secondary metabolism" laboratory course gives an introduction to the versatile soil dwelling Gram-positive bacteria Streptomyces and their secondary metabolism. The course combines genetic modification of Streptomyces; growing of the strain and protoplast preparation, plasmid isolation by alkaline lysis and phenol precipitation, digestions, and ligations prior to protoplast transformation, as well as investigating the secondary metabolites produced by the strains. Thus, the course is a combination of microbiology, molecular biology, and chemistry. After the course the students should understand the relationship between genes, proteins, and the produced metabolites. 2016 by The International Union of Biochemistry and Molecular Biology, 44(5):492-499, 2016.
2016 The International Union of Biochemistry and Molecular Biology.
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