A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä

A loss-of-function genetic screening identifies novel mediators of thyroid cancer cell viability




TekijätCantisani MC, Parascandolo A, Perala M, Allocca C, Fey V, Sahlberg N, Merolla F, Basolo F, Laukkanen MO, Kallioniemi OP, Santoro M, Castellone MD

KustantajaIMPACT JOURNALS LLC

Julkaisuvuosi2016

JournalOncotarget

Tietokannassa oleva lehden nimiONCOTARGET

Lehden akronyymiONCOTARGET

Vuosikerta7

Numero19

Aloitussivu28510

Lopetussivu28522

Sivujen määrä13

ISSN1949-2553

DOIhttps://doi.org/10.18632/oncotarget.8577


Tiivistelmä
RET, BRAF and other protein kinases have been identified as major molecular players in thyroid cancer. To identify novel kinases required for the viability of thyroid carcinoma cells, we performed a RNA interference screening in the RET/PTC1(CCDC6-RET)-positive papillary thyroid cancer cell line TPC1 using a library of synthetic small interfering RNAs (siRNAs) targeting the human kinome and related proteins. We identified 14 hits whose silencing was able to significantly reduce the viability and the proliferation of TPC1 cells; most of them were active also in BRAF-mutant BCPAP (papillary thyroid cancer) and 8505C (anaplastic thyroid cancer) and in RAS-mutant CAL62 (anaplastic thyroid cancer) cells. These included members of EPH receptor tyrosine kinase family as well as SRC and MAPK (mitogen activated protein kinases) families. Importantly, silencing of the identified hits did not affect significantly the viability of Nthy-ori 3-1 (hereafter referred to as NTHY) cells derived from normal thyroid tissue, suggesting cancer cell specificity. The identified proteins are worth exploring as potential novel druggable thyroid cancer targets.



Last updated on 2024-26-11 at 23:31