A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Flow-Tolerant Adhesion of a Bacterial Pathogen to Human Endothelial Cells Through Interaction With Biglycan
Tekijät: Salo Jemiina, Pietikäinen Annukka, Söderström Mirva, Auvinen Kaisa, Salmi Marko, Ebady Rhodaba, Moriarty Tara J, Viljanen Matti K, Hytönen Jukka
Kustantaja: OXFORD UNIV PRESS INC
Julkaisuvuosi: 2016
Journal: Journal of Infectious Diseases
Tietokannassa oleva lehden nimi: JOURNAL OF INFECTIOUS DISEASES
Lehden akronyymi: J INFECT DIS
Vuosikerta: 213
Numero: 100
Aloitussivu: 1623
Lopetussivu: 1631
Sivujen määrä: 9
ISSN: 0022-1899
eISSN: 1537-6613
DOI: https://doi.org/10.1093/infdis/jiw003
Tiivistelmä
Background. Bacterial pathogens causing systemic infections disseminate from the initial infection focus to the target organs usually through the blood vasculature. To be able to colonize various organs, bacteria need to adhere to the endothelial cells of the vascular wall, and the adhesion must be strong enough to resist the shear force of the blood flow. Borrelia burgdorferi sensu lato spirochetes, the causative agents of the tick- borne disease Lyme borreliosis, disseminate hematogenously from the tick bite site to the joints, the heart, and the central nervous system of the patient.Methods.aEuro integral We used both wild-type and genetically modified B. burgdorferi s. l. bacteria, recombinant borrelia adhesins, and an array of adhesion assays carried out both under stationary and flow conditions to investigate the molecular mechanisms of borrelial adhesion to human endothelial cells.Results.aEuro integral Borrelia garinii, a member of the B. burgdorferi s. l. complex, adhered to biglycan expressed by human endothelial cells in a flow-tolerant manner. The adhesion was mediated by the decorin-binding protein A (DbpA) and DbpB surface molecules of B. garinii.Conclusions.aEuro integral The proteoglycan biglycan is a receptor molecule for flow-resistant adhesion of the bacterial pathogen B. garinii on human endothelial cells.
Background. Bacterial pathogens causing systemic infections disseminate from the initial infection focus to the target organs usually through the blood vasculature. To be able to colonize various organs, bacteria need to adhere to the endothelial cells of the vascular wall, and the adhesion must be strong enough to resist the shear force of the blood flow. Borrelia burgdorferi sensu lato spirochetes, the causative agents of the tick- borne disease Lyme borreliosis, disseminate hematogenously from the tick bite site to the joints, the heart, and the central nervous system of the patient.Methods.aEuro integral We used both wild-type and genetically modified B. burgdorferi s. l. bacteria, recombinant borrelia adhesins, and an array of adhesion assays carried out both under stationary and flow conditions to investigate the molecular mechanisms of borrelial adhesion to human endothelial cells.Results.aEuro integral Borrelia garinii, a member of the B. burgdorferi s. l. complex, adhered to biglycan expressed by human endothelial cells in a flow-tolerant manner. The adhesion was mediated by the decorin-binding protein A (DbpA) and DbpB surface molecules of B. garinii.Conclusions.aEuro integral The proteoglycan biglycan is a receptor molecule for flow-resistant adhesion of the bacterial pathogen B. garinii on human endothelial cells.
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