Optimising of the transformation methods of black currant (Ribes nigrum L.) and development of transgenic resistance against black currant reversion virus

: Karjalainen R, Valimaki K, Pacot-Hiriart C, Kleemola M, Lehto K

: 2001

Acta Horticulturae

: PROCEEDINGS OF THE 4TH INTERNATIONAL SYMPOSIUM ON IN VITRO CULTURE AND HORTICULTURAL BREEDING

: ACTA HORTIC

: 560

: 169

: 172

: 4

: 90-6605-984-2

: 0567-7572

DOI: https://doi.org/10.17660/ActaHortic.2001.560.29

The effects of various growth hormones and their concentrations, different mediums and light conditions on the regeneration and transformation process of Finnish black currant varieties have been investigated. Agrobacterium-mediated and particle bombardment transformation methods have been tested. The optimised regeneration and transformation conditions are being established. Using the developed methods, we aim to transform black currant with genes conferring resistance to reversion disease. We have transferred several different versions of the coat protein gene (translatable, untranslatable, antisense) of the black currant reversion virus (BRV) into Nicotiana benthamiana host, and currently we are testing their resistance effect on this host. Based on these results, the fragment(s) giving the most effective resistance effect(s) will be selected and transferred into the black currant host.