A1 Refereed original research article in a scientific journal
Calcium impacts carbon and nitrogen balance in the filamentous cyanobacterium Anabaena sp PCC 7120
Authors: Walter J, Lynch F, Battchikova N, Aro EM, Gollan PJ
Publisher: OXFORD UNIV PRESS
Publication year: 2016
Journal: Journal of Experimental Botany
Journal name in source: JOURNAL OF EXPERIMENTAL BOTANY
Journal acronym: J EXP BOT
Volume: 67
Issue: 13
First page : 3997
Last page: 4008
Number of pages: 12
ISSN: 0022-0957
eISSN: 1460-2431
DOI: https://doi.org/10.1093/jxb/erw112(external)
Abstract
Calcium affects the primary cellular metabolism of Anabaena under conditions replete in both combined-nitrogen and inorganic carbon. Opposite transcriptome responses to calcium treatments occur for nitrogen- and carbon-related processes.Calcium is integral to the perception, communication and adjustment of cellular responses to environmental changes. However, the role of Ca2+ in fine-tuning cellular responses of wild-type cyanobacteria under favourable growth conditions has not been examined. In this study, extracellular Ca2+ has been altered, and changes in the whole transcriptome of Anabaena sp. PCC 7120 have been evaluated under conditions replete of carbon and combined nitrogen. Ca2+ induced differential expression of many genes driving primary cellular metabolism, with transcriptional regulation of carbon- and nitrogen-related processes responding with opposing trends. However, physiological effects of these transcriptional responses on biomass accumulation, biomass composition, and photosynthetic activity over the 24h period following Ca2+ adjustment were found to be minor. It is well known that intracellular carbon:nitrogen balance is integral to optimal cell growth and that Ca2+ plays an important role in the response of heterocystous cyanobacteria to combined-nitrogen deprivation. This work adds to the current knowledge by demonstrating a signalling role of Ca2+ for making sensitive transcriptional adjustments required for optimal growth under non-limiting conditions.
Calcium affects the primary cellular metabolism of Anabaena under conditions replete in both combined-nitrogen and inorganic carbon. Opposite transcriptome responses to calcium treatments occur for nitrogen- and carbon-related processes.Calcium is integral to the perception, communication and adjustment of cellular responses to environmental changes. However, the role of Ca2+ in fine-tuning cellular responses of wild-type cyanobacteria under favourable growth conditions has not been examined. In this study, extracellular Ca2+ has been altered, and changes in the whole transcriptome of Anabaena sp. PCC 7120 have been evaluated under conditions replete of carbon and combined nitrogen. Ca2+ induced differential expression of many genes driving primary cellular metabolism, with transcriptional regulation of carbon- and nitrogen-related processes responding with opposing trends. However, physiological effects of these transcriptional responses on biomass accumulation, biomass composition, and photosynthetic activity over the 24h period following Ca2+ adjustment were found to be minor. It is well known that intracellular carbon:nitrogen balance is integral to optimal cell growth and that Ca2+ plays an important role in the response of heterocystous cyanobacteria to combined-nitrogen deprivation. This work adds to the current knowledge by demonstrating a signalling role of Ca2+ for making sensitive transcriptional adjustments required for optimal growth under non-limiting conditions.
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