A1 Refereed original research article in a scientific journal
Molecular characterization of a satellite RNA associated with blackcurrant reversion nepovirus
Authors: Latvala-Kilby S, Lemmetty A, Lehto K
Publisher: SPRINGER-VERLAG WIEN
Publication year: 2000
Journal name in sourceARCHIVES OF VIROLOGY
Journal acronym: ARCH VIROL
Volume: 145
Issue: 1
First page : 51
Last page: 61
Number of pages: 11
ISSN: 0304-8608
DOI: https://doi.org/10.1007/s007050050004
Abstract
A satellite RNA (satRNA) associated with blackcurrant reversion nepovirus (BRV) was isolated and its nucleotide sequence was determined from cDNA clones. BRV satRNA was 1432 nucleotides (nt) in length excluding the poly(A)-tail, and contained one open reading frame which encodes a polypeptide of 402 amino acids, with a calculated M-r of 44 220. The coding region was bordered by a 5' leader sequence of 25 nt and a 3'-nontranslated region of 201 nt. Two in vitro translation products of approximately 45 kDa and 40 kDa were detected, indicating that two in-frame AUG codons at positions 26 and 134 may both be functional. Nucleotide sequence comparisons revealed a stretch of 865 nt that was 63% identical between BRV satRNA and the large satRNA of chicory yellow mottle nepovirus. A 5'-terminal consensus sequence and a 40 nt motif (located at positions 264-303 of BRV satRNA) were conserved between BRV satRNA and other nepoviral large satRNAs.
A satellite RNA (satRNA) associated with blackcurrant reversion nepovirus (BRV) was isolated and its nucleotide sequence was determined from cDNA clones. BRV satRNA was 1432 nucleotides (nt) in length excluding the poly(A)-tail, and contained one open reading frame which encodes a polypeptide of 402 amino acids, with a calculated M-r of 44 220. The coding region was bordered by a 5' leader sequence of 25 nt and a 3'-nontranslated region of 201 nt. Two in vitro translation products of approximately 45 kDa and 40 kDa were detected, indicating that two in-frame AUG codons at positions 26 and 134 may both be functional. Nucleotide sequence comparisons revealed a stretch of 865 nt that was 63% identical between BRV satRNA and the large satRNA of chicory yellow mottle nepovirus. A 5'-terminal consensus sequence and a 40 nt motif (located at positions 264-303 of BRV satRNA) were conserved between BRV satRNA and other nepoviral large satRNAs.
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