A1 Refereed original research article in a scientific journal
Hydrogen photoproduction by immobilized S-deprived Chlamydomonas reinhardtii: Effect of light intensity and spectrum, and initial medium pH
Authors: Taras K. Antal, Galina P. Kukarskikh, Alena A. Volgusheva, Tatyana E. Krendeleva,
Esa Tyystjärvi, Andrey B. Rubin
Publisher: ELSEVIER SCIENCE BV
Publication year: 2016
Journal: Algal Research
Journal name in source: ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
Journal acronym: ALGAL RES
Volume: 17
First page : 38
Last page: 45
Number of pages: 8
ISSN: 2211-9264
DOI: https://doi.org/10.1016/j.algal.2016.04.009
Abstract
Sulfur deprivation creates anaerobic conditions and thereby induces hydrogen photoproduction by the green microalga Chlamydomonas reinhardtii. In the present work we showed that immobilization of C. reinhardtii within a thin Ca-alginate film extended the duration of hydrogen evolution under sulfur depletion up to three weeks with hydrogen production limited mainly by exhaustion of intracellular starch reserves. The optimal incident light intensity for hydrogen photoproduction appeared to be about 80 mu mol photons m(-2) s(-1) at which the total amount of evolved hydrogen reached 0.8 mmol per mg chlorophyll. Illumination by monochromatic blue light peaking at 435 nm or red light with maximum at 690 nm, both favoring PSI excitation, improved the stability of PSII centers and enhanced hydrogen evolution. Initial medium pH in the range 6.5-7.0 was optimal for hydrogen photoproduction, likely due to enhanced accumulation of starch and elevated initial PSII activity. (C) 2016 Elsevier B.V. All rights reserved.
Sulfur deprivation creates anaerobic conditions and thereby induces hydrogen photoproduction by the green microalga Chlamydomonas reinhardtii. In the present work we showed that immobilization of C. reinhardtii within a thin Ca-alginate film extended the duration of hydrogen evolution under sulfur depletion up to three weeks with hydrogen production limited mainly by exhaustion of intracellular starch reserves. The optimal incident light intensity for hydrogen photoproduction appeared to be about 80 mu mol photons m(-2) s(-1) at which the total amount of evolved hydrogen reached 0.8 mmol per mg chlorophyll. Illumination by monochromatic blue light peaking at 435 nm or red light with maximum at 690 nm, both favoring PSI excitation, improved the stability of PSII centers and enhanced hydrogen evolution. Initial medium pH in the range 6.5-7.0 was optimal for hydrogen photoproduction, likely due to enhanced accumulation of starch and elevated initial PSII activity. (C) 2016 Elsevier B.V. All rights reserved.
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