A1 Journal article – refereed

5-Mercuricytosine: An Organometallic Janus Nucleobase




List of Authors: Ukale D, Shinde VS, Lönnberg T

Publisher: WILEY-V C H VERLAG GMBH

Publication year: 2016

Journal: Chemistry - A European Journal

Journal name in source: CHEMISTRY-A EUROPEAN JOURNAL

Journal acronym: CHEM-EUR J

Volume number: 22

Issue number: 23

Number of pages: 7

ISSN: 0947-6539

eISSN: 1521-3765

DOI: http://dx.doi.org/10.1002/chem.201600851


Abstract
The base-pairing properties of 5-mercuricytosine have been explored at the monomer level by NMR titrations and at the oligonucleotide level by melting temperature measurements. The NMR studies revealed a relatively high affinity for guanine, hypoxanthine, and uridine, that is, bases that are deprotonated upon coordination of Hg-II. Within an oligonucleotide duplex, 5-mercuricytosine formed Hg-II-mediated base pairs with thymine and guanine. In the former case, the duplex formed was as stable as the respective duplex comprising solely Watson-Crick base pairs. Based on detailed thermodynamic analysis of the melting curves, the stabilization by the Hg-II-mediated base pairs may be attributed to a comparatively low entropic penalty of hybridization.


Last updated on 2021-24-06 at 12:06