A1 Refereed original research article in a scientific journal

Anti-Inflammatory and Antioxidant Activity of Acalypha hispida Leaf and Analysis of its Major Bioactive Polyphenols by HPLC.



Authors: Md. Afjalus Siraj, Jamil A. Shilpi, Md. Golam Hossain, Shaikh Jamal Uddin, Md. Khirul Islam, Ismet Ara Jahan, Hemayet Hossain

Publishing place: Tabriz

Publication year: 2016

Journal: Advanced pharmaceutical bulletin

Journal name in source: Advanced pharmaceutical bulletin

Journal acronym: Adv Pharm Bull

Volume: 6

Issue: 2

First page : 275

Last page: 283

Number of pages: 9

ISSN: 2228-5881

eISSN: 2251-7308

DOI: https://doi.org/10.15171/apb.2016.039


Abstract
PURPOSE
Inflammation and oxidative stress can lead to different chronic diseases including cancer and atherosclerosis. Many medicinal plants have the potential to show as anti-inflammatory activity. Present investigation was performed to investigate anti-inflammatory, antioxidant activity, and quantification of selected bioactive plant polyphenols of the ethanol (EAH) and aqueous (AAH) extracts of Acalypha hispida (Euphorbiaceae) leaves.
METHODS
Anti-inflammatory activity was evaluated by carragenan and histamine induced rat paw edema models while antioxidant capacity was evaluated by DPPH free radical scavenging, Fe+2 chelating ability, reducing power, NO scavenging, total phenolic and total flavonoid content assay. Identification and quantification of bioactive polyphenols was done by HPLC.
RESULTS
At the doses of 200 and 400 mg/kg, both EAH and AAH showed statistically significant inhibition of paw volume in the anti-inflammatory activity test. Both the extracts showed DPPH scavenging (IC50: 14 and 17 µg/ml, respectively), Fe+2 ion chelating (IC50: 40 and 46 µg/ml, respectively), NO scavenging activity (65.49 and 60.66% inhibition at 100 µg/ml), and concentration dependent reducing power ability. For EAH and AAH, flavonoid content was 126.30 and 149.72 mg QE/g dry extract, while phenolic content was 130.51 and 173.80 mg GAE/g dry extract, respectively. HPLC analysis of EAH and AAH indicated the presence of high content of ellagic acid along with other phenolic constituents.
CONCLUSION
High content of ellagic acid along with other phenolic constituents might have played an important role in the observed anti-inflammatory and antioxidant activity.

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Last updated on 2024-26-11 at 23:13