A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Detection of the putative causal agent of blackcurrant reversion disease
Tekijät: Lemmetty A, Susi P, Latvala S, Lehto K
Julkaisuvuosi: 1998
Lehti:: Acta Horticulturae
Tietokannassa oleva lehden nimi: 8TH INTERNATIONAL SYMPOSIUM ON SMALL FRUIT VIRUS DISEASES
Lehden akronyymi: ACTA HORTIC
Numero: 471
Aloitussivu: 93
Lopetussivu: 98
Sivujen määrä: 6
ISBN: 90-6605-910-9
ISSN: 0567-7572
DOI: https://doi.org/10.17660/ActaHortic.1998.471.15
Tiivistelmä
The causal agent of blackcurrant reversion, a virus-like disease, has not yet been conclusively identified. Recently, we have isolated a new nepovirus from reverted blackcurrant, and partial characterization of the virus has provided materials for its rapid and sensitive detection in plants by IC-RT-PCR. Using this detection method, we have consistently associated the virus with the reversion disease, suggesting that it may be the causal agent of the disease. The virus is tentatively called, blackcurrant reversion associated virus, BRAV. We have tested detection of different isolates of the virus, originating from widely different geographic locations, by using four different primer pairs derived from the 3'-terminal sequence of a Finnish isolate of BRAV. One of the primer pairs amplifies the expected virus-specific fragments from all of the tested virus isolates, indicating that the viral sequences detected by this primer pair are well conserved in all isolates, including the common (E) and severe (R) forms of the reversion disease. Another primer pair amplifies the expected fragment from all but two of the tested isolates, and two other primer pairs amplify the expected fragments only from some of the tested isolates. The lack of detection of some virus isolates by these primers indicates that sequence variation commonly occurs in the areas of the binding sites of these primers. Sequence variation is also indicated by the results of the preliminary SSCP analysis of some of the PCR-amplified viral fragments.
The causal agent of blackcurrant reversion, a virus-like disease, has not yet been conclusively identified. Recently, we have isolated a new nepovirus from reverted blackcurrant, and partial characterization of the virus has provided materials for its rapid and sensitive detection in plants by IC-RT-PCR. Using this detection method, we have consistently associated the virus with the reversion disease, suggesting that it may be the causal agent of the disease. The virus is tentatively called, blackcurrant reversion associated virus, BRAV. We have tested detection of different isolates of the virus, originating from widely different geographic locations, by using four different primer pairs derived from the 3'-terminal sequence of a Finnish isolate of BRAV. One of the primer pairs amplifies the expected virus-specific fragments from all of the tested virus isolates, indicating that the viral sequences detected by this primer pair are well conserved in all isolates, including the common (E) and severe (R) forms of the reversion disease. Another primer pair amplifies the expected fragment from all but two of the tested isolates, and two other primer pairs amplify the expected fragments only from some of the tested isolates. The lack of detection of some virus isolates by these primers indicates that sequence variation commonly occurs in the areas of the binding sites of these primers. Sequence variation is also indicated by the results of the preliminary SSCP analysis of some of the PCR-amplified viral fragments.
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