Clamping of RNA with PNA enables targeting of microRNA.

: Ghidini A, Bergquist H, Murtola M, Punga T , Zain R, Strömberg R

Publisher: ROYAL SOC CHEMISTRY

: 2016

Organic and Biomolecular Chemistry

: Organic & biomolecular chemistry

: Org Biomol Chem

: 14

: 23

: 5210

: 5213

: -5206

: 1477-0539

DOI: https://doi.org/10.1039/c6ob00516k

To be able to target microRNAs also at stages where these are in a double stranded or hairpin form we have studied BisPNA designed to clamp the target and give sufficient affinity to allow for strand invasion. We show that BisPNA complexes are more stable with RNA than with DNA. In addition, 24-mer BisPNA (AntimiR) constructs form complexes with a hairpin RNA that is a model of the microRNA miR-376b, suggesting that PNA-clamping may be an effective way of targeting microRNAs.