Oligonucleotide Array-in-Well Platform for Detection and Genotyping Human Adenoviruses by Utilizing Upconverting Phosphor Label Technology

: Ylihärsilä M, Valta T, Karp M, Hattara L, Harju E, Hölsä J, Saviranta P, Waris M, Soukka T

Publisher: AMER CHEMICAL SOC

: 2011

: Analytical Chemistry

: ANALYTICAL CHEMISTRY

: ANAL CHEM

: 4

: 83

: 4

: 1456

: 1461

: 6

: 0003-2700

DOI: https://doi.org/10.1021/ac103155f

We have developed a robust array-in-well test platform based on an oligonudeotide array, combining advantages of simple instrumentation and new upconverting phosphor reporter technology. Upconverting inorganic lanthanide phosphors have a unique property of photoluminescence emission at visible wavelengths under near-infrared excitation. No autofluorescence is produced from the sample or support material, enabling a highly sensitive assay. In this study, the assay is performed in standard 96-well microtiter plates, making the technique easily adaptable to high-throughput analysis. The oligonucleotide array-in-well assay is employed to detect a selection of ten common adenovirus genotypes causing human infections. The study provides a demonstration of the advantages and potential of the upconverting phosphor-based reporter technology in multianalyte assays and anti-Stokes photoluminescence detection with an anti-Stokes photoluminescence imaging device.