A1 Refereed original research article in a scientific journal
Thiolation and Cell-Penetrating Peptide Surface Functionalization of Porous Silicon Nanoparticles for Oral Delivery of Insulin
Authors: Shrestha N, Araujo F, Shahbazi MA, Makila E, Gomes MJ, Herranz-Blanco B, Lindgren R, Granroth S, Kukk E, Salonen J, Hirvonen J, Sarmento B, Santos HA
Publisher: WILEY-V C H VERLAG GMBH
Publication year: 2016
Journal: Advanced Functional Materials
Journal name in source: ADVANCED FUNCTIONAL MATERIALS
Journal acronym: ADV FUNCT MATER
Volume: 26
Issue: 20
First page : 3405
Last page: 3416
Number of pages: 12
ISSN: 1616-301X
eISSN: 1616-3028
DOI: https://doi.org/10.1002/adfm.201505252(external)
Abstract
During the last decades, advanced oral delivery systems to enhance the intestinal absorption of widely applicable proteins and peptides, particularly insulin, have been developed. Here, chitosan-conjugated undecylenic acid-modified thermally hydrocarbonized porous silicon nanoparticles (CSUn NPs) are used, which promote the mucoadhesion and cellular interactions, thus boosting the intestinal permeability of insulin. Then, to further potentiate the mucoadhesion and permeability enhancement of chitosan-modified NPs, the surface of the NPs is further modified with either L-cysteine (CYS-CSUn NPs) or a cell-penetrating peptide (CPP-CSUn NPs). CYS-CSUn and CPP-CSUn NPs show 17- and 12-fold increase in the apparent permeability of insulin across cellular intestinal cells, respectively, with significant enhancement in the cellular interactions. The insulin uptake mechanism pathways in intestinal cells from the developed NPs are also unraveled, which demonstrates major involvement of active transport process and electrostatic interactions, along with adsorptive and clathrin-mediated endocytic pathways. Moreover, after oral administration in diabetic rats, CYS-CSUn NPs show 1.86- and 2.03-fold increase in the relative bioavailability of insulin, as compared to empty NPs and oral insulin solution, respectively. In conclusion, this study presents L-cysteine modified CSUn NPs as a promising strategy with the ability to overcome the multiple barriers for oral delivery of insulin.
During the last decades, advanced oral delivery systems to enhance the intestinal absorption of widely applicable proteins and peptides, particularly insulin, have been developed. Here, chitosan-conjugated undecylenic acid-modified thermally hydrocarbonized porous silicon nanoparticles (CSUn NPs) are used, which promote the mucoadhesion and cellular interactions, thus boosting the intestinal permeability of insulin. Then, to further potentiate the mucoadhesion and permeability enhancement of chitosan-modified NPs, the surface of the NPs is further modified with either L-cysteine (CYS-CSUn NPs) or a cell-penetrating peptide (CPP-CSUn NPs). CYS-CSUn and CPP-CSUn NPs show 17- and 12-fold increase in the apparent permeability of insulin across cellular intestinal cells, respectively, with significant enhancement in the cellular interactions. The insulin uptake mechanism pathways in intestinal cells from the developed NPs are also unraveled, which demonstrates major involvement of active transport process and electrostatic interactions, along with adsorptive and clathrin-mediated endocytic pathways. Moreover, after oral administration in diabetic rats, CYS-CSUn NPs show 1.86- and 2.03-fold increase in the relative bioavailability of insulin, as compared to empty NPs and oral insulin solution, respectively. In conclusion, this study presents L-cysteine modified CSUn NPs as a promising strategy with the ability to overcome the multiple barriers for oral delivery of insulin.
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