A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Lipid-based Nutrient Supplements Do Not Affect Gut Bifidobacterium Microbiota in Malawian Infants: a Randomised Trial
Tekijät: Juhani Aakko, Łukasz Grześkowiak, Tiia Asukas, Eerika Päivänsäde, Kirsi-Maarit Lehto, Yue-Mei Fan, Charles
Mangani, Kenneth Maleta, Per Ashorn, Seppo Salminen
Julkaisuvuosi: 2017
Journal: Journal of Pediatric Gastroenterology and Nutrition
Tietokannassa oleva lehden nimi: Journal of pediatric gastroenterology and nutrition
Lehden akronyymi: J Pediatr Gastroenterol Nutr
Vuosikerta: 64
Numero: 4
Aloitussivu: 610
Lopetussivu: 615
Sivujen määrä: 6
ISSN: 1536-4801
DOI: https://doi.org/10.1097/MPG.0000000000001333
Tiivistelmä
OBJECTIVE
To assess the effect of nutritional supplementation with lipid-based nutrient supplements (LNS) and corn-soy blend (CSB) flour on Bifidobacterium and Staphylococcus aureus gut microbiota composition in Malawian infants. In addition, the microbiota changes over time were characterized in the study infants.
METHODS
Healthy six-month-old Malawian infants were randomly assigned to one of four intervention schemes for a six-month period. Infants in the control group were not provided with any supplementary food. Infants in other three groups received either micronutrient-fortified CSB, micronutrient-fortified LNS with milk protein base or micronutrient-fortified LNS with soy protein base between six and twelve months of age. Fecal bifidobacteria and Staphylococcus aureus gut microbiota at six and twelve months of age was analyzed by qPCR method.
RESULTS
There was no difference in change in bacterial prevalence or counts between the intervention groups over the 6-month study period. When looking at the total study population, higher counts of total bacteria (p = 0.028), Bifidobacterium genus (p = 0.027), B. catenulatum (p = 0.031) and lower counts of B. infantis (p < 0.001), B. lactis (p < 0.001), B. longum (p < 0.001) and S. aureus (p < 0.001) were detected in the children's stools at 12 months rather than at 6 months of age.
CONCLUSIONS
The dietary supplementation did not have an impact on the Bifidobacterium and Staphylococcus aureus microbiota composition of the study infants. However, the fecal bifidobacterial diversity of the infants changed towards a more adult-like microbiota profile within the observed time.
OBJECTIVE
To assess the effect of nutritional supplementation with lipid-based nutrient supplements (LNS) and corn-soy blend (CSB) flour on Bifidobacterium and Staphylococcus aureus gut microbiota composition in Malawian infants. In addition, the microbiota changes over time were characterized in the study infants.
METHODS
Healthy six-month-old Malawian infants were randomly assigned to one of four intervention schemes for a six-month period. Infants in the control group were not provided with any supplementary food. Infants in other three groups received either micronutrient-fortified CSB, micronutrient-fortified LNS with milk protein base or micronutrient-fortified LNS with soy protein base between six and twelve months of age. Fecal bifidobacteria and Staphylococcus aureus gut microbiota at six and twelve months of age was analyzed by qPCR method.
RESULTS
There was no difference in change in bacterial prevalence or counts between the intervention groups over the 6-month study period. When looking at the total study population, higher counts of total bacteria (p = 0.028), Bifidobacterium genus (p = 0.027), B. catenulatum (p = 0.031) and lower counts of B. infantis (p < 0.001), B. lactis (p < 0.001), B. longum (p < 0.001) and S. aureus (p < 0.001) were detected in the children's stools at 12 months rather than at 6 months of age.
CONCLUSIONS
The dietary supplementation did not have an impact on the Bifidobacterium and Staphylococcus aureus microbiota composition of the study infants. However, the fecal bifidobacterial diversity of the infants changed towards a more adult-like microbiota profile within the observed time.
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