A1 Refereed original research article in a scientific journal
Evaluation of immunohistochemistry and in situ hybridization methods for the detection of enteroviruses using infected cell culture samples
Authors: Oikarinen M, Tauriainen S, Penttila P, Keim J, Rantala I, Honkanen T, Hyoty H
Publisher: ELSEVIER SCIENCE BV
Publication year: 2010
Journal: Journal of Clinical Virology
Journal name in source: JOURNAL OF CLINICAL VIROLOGY
Journal acronym: J CLIN VIROL
Volume: 47
Issue: 3
First page : 224
Last page: 228
Number of pages: 5
ISSN: 1386-6532
DOI: https://doi.org/10.1016/j.jcv.2009.12.020
Abstract
Background: Enterovirus infections are frequent in all age groups. In addition to acute infections, they
have been connected to chronic diseases such as cardiomyopathies and type 1 diabetes. Based on this
there is an increasing need for the reliable detection of enteroviruses in different kinds of tissue samples.
Objectives: The aim of this study was to set up a test panel which can detect a wide range of different
enteroviruses in paraffin-embedded samples and fresh frozen samples using immunohistochemical and
in situ hybridization methods.
Study design: A panel of nine enterovirus antibodies was optimized for the detection of different
enterovirus types in both paraffin-embedded and frozen cell culture samples. In addition, an
oligonucleotide probe detecting allhumanenteroviruses was evaluated for ISH in formalin-fixed paraffinembedded
cell culture samples.
Results: Most antibodies worked well in both sample types. Some antibodies detected only one of the
tested serotypes, whereas others detected several serotypes. ISH was able to detect all tested enterovirus
types.
Conclusions: This test panel makes it possible to detect a wide range of different enterovirus types in both
formalin-fixed paraffin-embedded and frozen samples. The same methods can also be applied for tissue
sections, but may need further optimization for each tissue type.
Background: Enterovirus infections are frequent in all age groups. In addition to acute infections, they
have been connected to chronic diseases such as cardiomyopathies and type 1 diabetes. Based on this
there is an increasing need for the reliable detection of enteroviruses in different kinds of tissue samples.
Objectives: The aim of this study was to set up a test panel which can detect a wide range of different
enteroviruses in paraffin-embedded samples and fresh frozen samples using immunohistochemical and
in situ hybridization methods.
Study design: A panel of nine enterovirus antibodies was optimized for the detection of different
enterovirus types in both paraffin-embedded and frozen cell culture samples. In addition, an
oligonucleotide probe detecting allhumanenteroviruses was evaluated for ISH in formalin-fixed paraffinembedded
cell culture samples.
Results: Most antibodies worked well in both sample types. Some antibodies detected only one of the
tested serotypes, whereas others detected several serotypes. ISH was able to detect all tested enterovirus
types.
Conclusions: This test panel makes it possible to detect a wide range of different enterovirus types in both
formalin-fixed paraffin-embedded and frozen samples. The same methods can also be applied for tissue
sections, but may need further optimization for each tissue type.
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