A1 Refereed original research article in a scientific journal
Susceptibility of human tonsillar epithelial cells to enterovirus 71 with normal cytokine response
Authors: Xie GC, Guo NJ, Grénman R, Wang H, Wang Y, Vuorenmma M, Zhang Q, Zhang S, Li HY, Pang LL, Li DD, Jin M, Sun XM, Kong XY, Duan ZJ
Publication year: 2016
Journal: Virology
Volume: 494
First page : 108
Last page: 118
Number of pages: 11
ISSN: 0042-6822
DOI: https://doi.org/10.1016/j.virol.2016.04.016
A recent histopathologic study implicated human tonsillar crypt
epithelium as an important site for EV71 replication in EV71-caused
fatal cases. This study aimed to confirm the susceptibility of human
tonsillar epithelium to EV71. Two human tonsillar epithelial cell lines
(UT-SCC-60A and UT-SCC-60B) were susceptive to EV71, and PI3K/AKT, p38,
ERK1/2, and JNK1/2 signal pathways were activated. Interferon-α, IL-8,
IL-1β, IL-6 and IL-12p40 were induced and regulated by PI3K/AKT, p38,
ERK1/2, and JNK1/2 signal pathways. PI3K/AKT pathway activation appeared
to suppress the induction of TNF-α, which induced cell survival by
inhibiting GSK-3β. The activation of NF-κB was observed but inhibited by
these pathways in EV71 infection. Furthermore, ERK1/2 and JNK1/2 were
essential for efficient EV71 replication. Human tonsillar epithelial
cells support EV71 replication and display innate antiviral immunity in vitro, indicating that human tonsillar epithelial cells may be novel targets for EV71 infection and replication in vivo.
