A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
HPLC-PDA-ESI-MS/MS profiling and chemopreventive potential of Eucalyptus gomphocephala DC
Tekijät: Al-Sayed E, Singab AN, Ayoub N, Martiskainen O, Sinkkonen J, Pihlaja K
Kustantaja: ELSEVIER SCI LTD
Julkaisuvuosi: 2012
Journal: Food Chemistry
Tietokannassa oleva lehden nimi: FOOD CHEMISTRY
Lehden akronyymi: FOOD CHEM
Numero sarjassa: 3
Vuosikerta: 133
Numero: 3
Aloitussivu: 1017
Lopetussivu: 1024
Sivujen määrä: 8
ISSN: 0308-8146
DOI: https://doi.org/10.1016/j.foodchem.2011.09.036
Tiivistelmä
An HPLC-PDA-ESI/MS/MS method was developed to identify the phytoconstituents of the EtOAc fraction of Eucalyptus gomphocephala DC. The antioxidant effect of the EtOAc fraction together with its sub-fractions was determined in vitro. The cytotoxicity was evaluated on different cell lines. The EtOAc fraction exhibited strong antioxidant activity, reduced the viability of all cell lines and was more active on MCF-7 and HepG-2 cell lines. Subsequently, the cytotoxicity of the sub-fractions and the isolated compounds were tested on MCF-7, HepG-2. The EtOAc fraction possessed potential antitumour promoting properties. It inhibited the stimulated NO (20%), 5-LOX (48.0%) and COX-2 (49.7%) respectively (at concentration of 20 mu g/ml). This study suggests that this fraction is a source of different antioxidant and cytotoxic compounds with potential chemopreventive properties that might prevent different stages of the carcinogenesis process. (C), 2011 Elsevier Ltd. All rights reserved.
An HPLC-PDA-ESI/MS/MS method was developed to identify the phytoconstituents of the EtOAc fraction of Eucalyptus gomphocephala DC. The antioxidant effect of the EtOAc fraction together with its sub-fractions was determined in vitro. The cytotoxicity was evaluated on different cell lines. The EtOAc fraction exhibited strong antioxidant activity, reduced the viability of all cell lines and was more active on MCF-7 and HepG-2 cell lines. Subsequently, the cytotoxicity of the sub-fractions and the isolated compounds were tested on MCF-7, HepG-2. The EtOAc fraction possessed potential antitumour promoting properties. It inhibited the stimulated NO (20%), 5-LOX (48.0%) and COX-2 (49.7%) respectively (at concentration of 20 mu g/ml). This study suggests that this fraction is a source of different antioxidant and cytotoxic compounds with potential chemopreventive properties that might prevent different stages of the carcinogenesis process. (C), 2011 Elsevier Ltd. All rights reserved.
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