A1 Refereed original research article in a scientific journal
Pancreatic phospholipase A2 contributes to lung injury in experimental meconium aspiration
Authors: Sippola T., Aho H., Peuravuori H., Lukkarinen H., Gunn J., Kääpä P.
Publisher: INT PEDIATRIC RESEARCH FOUNDATION, INC
Publication year: 2006
Journal: Pediatric Research
Journal name in source: Pediatric Research
Journal acronym: PEDIATR RES
Volume: 59
Issue: 5
First page : 641
Last page: 645
Number of pages: 5
ISSN: 0031-3998
DOI: https://doi.org/10.1203/01.pdr.0000214685.31232.6a(external)
Abstract
To investigate the role of pancreatic (group I) secretory PLA (sPLA-I) in the pathogenesis of meconium aspiration syndrome, human particulate meconium or its supernatant either before or after extraction of PLA-I was insufflated into rat lungs. In addition, the pulmonary effects of intra-tracheal human and bovine PLA-I were studied. Lungs with saline instillation served as controls. Intrapulmonary particulate meconium (both before and after PLA-I extraction), unlike meconium supernatant, resulted in markedly elevated lung tissue PLA catalytic activity and human PLA-I concentrations when compared with controls. On the other hand, tissue concentrations of the group II PLA remained unchanged in all meconium lungs. Pulmonary PLA -I concentrations further correlated positively with lung injury scores. Instillation of meconium-derived human PLA-I, at a concentration of one-third of that in particulate meconium, did not raise PLA activity or concentrations of PLA-I or PLA -II in the lung tissue from the control level, but still resulted in significantly elevated lung wet/diy ratio and injury score. In contrast, insufflation of bovine pancreatic PLA increased the lung tissue enzyme activity and wet/dry ratio from the control level, but had no effect on the type II PLA concentration or lung injury score. Our data thus indicate that human pancreatic PLA, introduced in high amounts within aspirated meconium especially in particulate form, is a potent inducer of lung tissue inflammatory injury. Copyright © 2006 International Pediatric Research Foundation, Inc.
To investigate the role of pancreatic (group I) secretory PLA (sPLA-I) in the pathogenesis of meconium aspiration syndrome, human particulate meconium or its supernatant either before or after extraction of PLA-I was insufflated into rat lungs. In addition, the pulmonary effects of intra-tracheal human and bovine PLA-I were studied. Lungs with saline instillation served as controls. Intrapulmonary particulate meconium (both before and after PLA-I extraction), unlike meconium supernatant, resulted in markedly elevated lung tissue PLA catalytic activity and human PLA-I concentrations when compared with controls. On the other hand, tissue concentrations of the group II PLA remained unchanged in all meconium lungs. Pulmonary PLA -I concentrations further correlated positively with lung injury scores. Instillation of meconium-derived human PLA-I, at a concentration of one-third of that in particulate meconium, did not raise PLA activity or concentrations of PLA-I or PLA -II in the lung tissue from the control level, but still resulted in significantly elevated lung wet/diy ratio and injury score. In contrast, insufflation of bovine pancreatic PLA increased the lung tissue enzyme activity and wet/dry ratio from the control level, but had no effect on the type II PLA concentration or lung injury score. Our data thus indicate that human pancreatic PLA, introduced in high amounts within aspirated meconium especially in particulate form, is a potent inducer of lung tissue inflammatory injury. Copyright © 2006 International Pediatric Research Foundation, Inc.
UTU Research Portal