A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Endothelial amine oxidase AOC3 transiently contributes to adaptive immune responses in the airways
Tekijät: Johannes Dunkel, Juan Antonio Aguilar-Pimentel, Markus Ollert, Helmut Fuchs, Valerie Gailus-Durner, Martin Hrabˇe de Angelis, Sirpa Jalkanen, Marko Salmi, Tibor Z. Veres
Kustantaja: Wiley-VCH Verlag GmbH & Co KGaA
Kustannuspaikka: Weinheim
Julkaisuvuosi: 2014
Journal: European Journal of Immunology
Tietokannassa oleva lehden nimi: EUROPEAN JOURNAL OF IMMUNOLOGY
Lehden akronyymi: EUR J IMMUNOL
Vuosikerta: 44
Numero: 11
Aloitussivu: 3232
Lopetussivu: 3239
Sivujen määrä: 8
ISSN: 0014-2980
eISSN: 1521-4141
DOI: https://doi.org/10.1002/eji.201444563
Amine oxidase, copper containing 3 (AOC3, also known as vascular adhesion protein-1 (VAP-1)) is an endothelial adhesion molecule that contributes to the extravasation of neutrophils, macrophages, and lymphocytes to sites of inflammation. However, the role of AOC3/VAP-1 in allergic responses remains unknown. Here, we studied eosinophil and CD4(+) T-cell recruitment to the airways using AOC3/VAP-1-deficient mice. In an OVA-triggered asthma model, AOC3/VAP-1 slightly contributed to the accumulation of leukocytes in lungs in an age-dependent manner. We then established a new model to kinetically measure recruitment of OVA-specific CD4(+) T cells to different airway immune compartments during the priming and effector phases of an adaptive immune response. The results showed that in the absence of AOC3/VAP-1, recruitment of antigen-specific CD4(+) T cells to draining bronchial lymph nodes is reduced by 89% on day 3 after tracheal allergen exposure, but this difference was not observed on day 6. The dispersal of effector cells to lung and tracheal mucosa is AOC3/VAP-1 independent. Thus, in allergic airway reactions, AOC3/VAP-1 transiently contributes to the antigen-specific, CD4(+) T-cell traffic to secondary lymphatic tissues, but not to airway mucosa or lung parenchyma. Our results suggest a largely redundant function for AOC3/VAP-1 in allergic inflammatory responses of the airways.