Double-restriction-site-associated DNA (dRAD) approach for fast microsatellite marker development in Eurasian perch (Perca fluviatilis L.)



: Lilian Pukk, Veljo Kisand, Freed Ahmad, Riho Gross, Anti Vasemägi

Publisher: SPRINGER

: 2014

 Conservation Genetics Resources

: CONSERVATION GENETICS RESOURCES

: CONSERV GENET RESOUR

: 6

: 1

: 183

: 184

: 2

: 1877-7252

DOI: https://doi.org/10.1007/s12686-013-0042-2


Seventeen polymorphic microsatellite markers were developed for Eurasian perch, using double-restriction-site-associated DNA approach combined with Ion Torrent PGM (TM) sequencing. In test panel of 48 individuals from two populations, the average number of alleles per locus was 10.2, ranging from 2 to 24. These variable microsatellites will be invaluable resource for population genetic studies in perch.




dRAD tag, Eurasian perch (Perca fluviatilis L.), Genome complexity reduction, Ion Torrent PGM (TM) sequencing, Microsatellites, Next generation sequencing



Last updated on 26/11/2024 05:50:58 PM