Double-restriction-site-associated DNA (dRAD) approach for fast microsatellite marker development in Eurasian perch (Perca fluviatilis L.) - UTU Research Portal

A1 Refereed original research article in a scientific journal

Double-restriction-site-associated DNA (dRAD) approach for fast microsatellite marker development in Eurasian perch (Perca fluviatilis L.)

Authors: Lilian Pukk, Veljo Kisand, Freed Ahmad, Riho Gross, Anti Vasemägi

Publisher: SPRINGER

Publication year: 2014

Journal: Conservation Genetics Resources

Journal name in source: CONSERVATION GENETICS RESOURCES

Journal acronym: CONSERV GENET RESOUR

Volume: 6

Issue: 1

First page : 183

Last page: 184

Number of pages: 2

ISSN: 1877-7252

DOI: https://doi.org/10.1007/s12686-013-0042-2(external)

Abstract

Seventeen polymorphic microsatellite markers were developed for Eurasian perch, using double-restriction-site-associated DNA approach combined with Ion Torrent PGM (TM) sequencing. In test panel of 48 individuals from two populations, the average number of alleles per locus was 10.2, ranging from 2 to 24. These variable microsatellites will be invaluable resource for population genetic studies in perch.