A1 Refereed original research article in a scientific journal
Extended H-2 photoproduction by N-2-fixing cyanobacteria immobilized in thin alginate films
Authors: Leino H, Kosourov SN, Saari L, Sivonen K, Tsygankov AA, Aro EM, Allahverdiyeva Y
Publisher: PERGAMON-ELSEVIER SCIENCE LTD
Publication year: 2012
Journal: International Journal of Hydrogen Energy
Journal name in source: INTERNATIONAL JOURNAL OF HYDROGEN ENERGY
Journal acronym: INT J HYDROGEN ENERG
Number in series: 1
Volume: 37
Issue: 1
First page : 151
Last page: 161
Number of pages: 11
ISSN: 0360-3199
DOI: https://doi.org/10.1016/j.ijhydene.2011.09.088
Abstract
Screening of the University of Helsinki Culture Collection for naturally good H-2 producing cyanobacteria recently revealed several promising strains. One of the superior strains is Calothrix 336/3, an N-2-fixing heterocystous filamentous cyanobacterium. Making use of an important feature of the Calothrix 336/3 cells to adhere to the substrate, we applied an immobilization technique to improve H-2 production capacity of this strain. We examined the basic properties of immobilization in Ca2+-alginate films in response to the production of H-2 of the Calothrix 336/3 strain and as reference strains we used a model organism Anabaena PCC 7120 and its uptake hydrogenase mutant, Delta hupL, that allow us to compare the responses of different strains to alginate entrapment. Immobilization of the Calothrix 336/3 and Delta hupL mutant cells in Ca2+-alginate resulted in prolonged H-2 production over several cycles. Immobilization of the Calothrix 336/3 cells was most successful and production of H-2 could be measured even after 40 days after immobilization. Copyright (C) 2011, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
Screening of the University of Helsinki Culture Collection for naturally good H-2 producing cyanobacteria recently revealed several promising strains. One of the superior strains is Calothrix 336/3, an N-2-fixing heterocystous filamentous cyanobacterium. Making use of an important feature of the Calothrix 336/3 cells to adhere to the substrate, we applied an immobilization technique to improve H-2 production capacity of this strain. We examined the basic properties of immobilization in Ca2+-alginate films in response to the production of H-2 of the Calothrix 336/3 strain and as reference strains we used a model organism Anabaena PCC 7120 and its uptake hydrogenase mutant, Delta hupL, that allow us to compare the responses of different strains to alginate entrapment. Immobilization of the Calothrix 336/3 and Delta hupL mutant cells in Ca2+-alginate resulted in prolonged H-2 production over several cycles. Immobilization of the Calothrix 336/3 cells was most successful and production of H-2 could be measured even after 40 days after immobilization. Copyright (C) 2011, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.
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