A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Localization of the P1 protein of potato Y potyvirus in association with cytoplasmic inclusion bodies and in the cytoplasm of infected cells
Tekijät: Arbatova J, Lehto K, Pehu E, Pehu T
Kustantaja: SOC GENERAL MICROBIOLOGY
Julkaisuvuosi: 1998
Lehti:: Journal of General Virology
Tietokannassa oleva lehden nimi: JOURNAL OF GENERAL VIROLOGY
Lehden akronyymi: J GEN VIROL
Vuosikerta: 79
Aloitussivu: 2319
Lopetussivu: 2323
Sivujen määrä: 5
ISSN: 0022-1317
DOI: https://doi.org/10.1099/0022-1317-79-10-2319
Tiivistelmä
The N-terminal P1 proteinase of potato virus Y (ordinary strain group isolate PVY-O) was expressed in E, coli. Antiserum was raised against the expressed protein and used to detect the viral proteins in infected tobacco leaf tissue by Western blotting and by electron microscopy with immunogold labelling. In the immunogold localization studies P1 protein was detected in association with the cytoplasmic inclusion bodies characteristic of PVY infections and in the cytoplasm of the infected plant cells. No significant P1 antibody binding with other plant cell organelles, or with the cell wall and plasmodesmata, was detected by immunogold labelling.
The N-terminal P1 proteinase of potato virus Y (ordinary strain group isolate PVY-O) was expressed in E, coli. Antiserum was raised against the expressed protein and used to detect the viral proteins in infected tobacco leaf tissue by Western blotting and by electron microscopy with immunogold labelling. In the immunogold localization studies P1 protein was detected in association with the cytoplasmic inclusion bodies characteristic of PVY infections and in the cytoplasm of the infected plant cells. No significant P1 antibody binding with other plant cell organelles, or with the cell wall and plasmodesmata, was detected by immunogold labelling.
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