A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Effects of beta-carotene and retinal on the formation of methyl linoleate hydroperoxides
Tekijät: Haila K, Hopia A, Heinonen M
Kustantaja: WILEY-BLACKWELL
Julkaisuvuosi: 2000
Lehti:: European Journal of Lipid Science and Technology
Tietokannassa oleva lehden nimi: EUROPEAN JOURNAL OF LIPID SCIENCE AND TECHNOLOGY
Lehden akronyymi: EUR J LIPID SCI TECH
Vuosikerta: 102
Numero: 1
Aloitussivu: 31
Lopetussivu: 36
Sivujen määrä: 6
ISSN: 1438-7697
Tiivistelmä
In order to clarify the prooxidative role of carotenoids on the oxidation of unsaturated lipids this study examined the effects of beta-carotene and its oxidative breakdown product, retinal, on primary oxidation products of linoleic acid methyl ester. Formation as well as isomer distribution of methyl linoleate hydroperoxides were followed by highperformance liquid chromatography. Oxidation of methyl linoleate without or with added beta-carotene (5, 20, 200 mu g/g) or retinal (7, 18, 180, 360 mu g/g) was carried out in the dark under air at 40 degrees C. Both beta-carotene and retinal promoted the formation of hy droperoxides and thus acted as prooxidants in a concentration-dependent way. Moreover, carotenoids also had an effect on the isomeric distribution of primary oxidation products as high contents of retinal increased the portion (%) of trans,trans-hydroperoxides. Being thermodynamically more stable isomers than cis,trans-isomers of hydroperoxides they are known to accumulate during later phases of oxidation or during hydroperoxide decomposition. The results showed that beta-carotene and retinal were not effective hydrogen donors. These findings raise the question that carotenoids and their oxidative breakdown products enhance the decomposition of lipid hydroperoxides and this effect partially explains the prooxidative effect of carotenoids.
In order to clarify the prooxidative role of carotenoids on the oxidation of unsaturated lipids this study examined the effects of beta-carotene and its oxidative breakdown product, retinal, on primary oxidation products of linoleic acid methyl ester. Formation as well as isomer distribution of methyl linoleate hydroperoxides were followed by highperformance liquid chromatography. Oxidation of methyl linoleate without or with added beta-carotene (5, 20, 200 mu g/g) or retinal (7, 18, 180, 360 mu g/g) was carried out in the dark under air at 40 degrees C. Both beta-carotene and retinal promoted the formation of hy droperoxides and thus acted as prooxidants in a concentration-dependent way. Moreover, carotenoids also had an effect on the isomeric distribution of primary oxidation products as high contents of retinal increased the portion (%) of trans,trans-hydroperoxides. Being thermodynamically more stable isomers than cis,trans-isomers of hydroperoxides they are known to accumulate during later phases of oxidation or during hydroperoxide decomposition. The results showed that beta-carotene and retinal were not effective hydrogen donors. These findings raise the question that carotenoids and their oxidative breakdown products enhance the decomposition of lipid hydroperoxides and this effect partially explains the prooxidative effect of carotenoids.
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