A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä
Molecular analysis of the carbapenem and metronidazole resistance mechanisms of Bacteroides strains reported in a Europe-wide antibiotic resistance survey
Tekijät: Soki J, Eitel Z, Urban E, Nagy E, on behalf of the ESCMID Study Group on Anaerobic Infections (incl. Könönen E)
Kustantaja: ELSEVIER SCIENCE BV
Julkaisuvuosi: 2013
Lehti: International Journal of Antimicrobial Agents
Tietokannassa oleva lehden nimi: INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
Lehden akronyymi: INT J ANTIMICROB AG
Numero sarjassa: 2
Vuosikerta: 41
Numero: 2
Aloitussivu: 122
Lopetussivu: 125
Sivujen määrä: 4
ISSN: 0924-8579
DOI: https://doi.org/10.1016/j.ijantimicag.2012.10.001
Tiivistelmä
Here we examine the carbapenem and metronidazole resistance mechanisms of 640 Bacteroides strains reported in the 2008-2009 European antibiotic susceptibility survey. Of the 22 strains with elevated imipenem minimum inhibitory concentrations (>4 mu g/mL), 10 were cfiA-positive and out of these 5 carried activating insertion sequence (IS) elements in the upstream regions of the cfiA genes. However, resistant strains with cfiA genes but with no activating IS elements were found (n = 2) as well as a resistant strain with no cfiA gene. In the former the resistance phenotypes by Etest were heterogeneous, whilst in the latter no carbapenemase production was seen; both mechanisms have been rarely observed, examined and characterised. Interestingly, few (n = 3) nim-positive strains were found, including one metronidazole-resistant strain harbouring nimE activated by ISBf6, and two susceptible strains harbouring chromosomally located nim genes. (C) 2012 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
Here we examine the carbapenem and metronidazole resistance mechanisms of 640 Bacteroides strains reported in the 2008-2009 European antibiotic susceptibility survey. Of the 22 strains with elevated imipenem minimum inhibitory concentrations (>4 mu g/mL), 10 were cfiA-positive and out of these 5 carried activating insertion sequence (IS) elements in the upstream regions of the cfiA genes. However, resistant strains with cfiA genes but with no activating IS elements were found (n = 2) as well as a resistant strain with no cfiA gene. In the former the resistance phenotypes by Etest were heterogeneous, whilst in the latter no carbapenemase production was seen; both mechanisms have been rarely observed, examined and characterised. Interestingly, few (n = 3) nim-positive strains were found, including one metronidazole-resistant strain harbouring nimE activated by ISBf6, and two susceptible strains harbouring chromosomally located nim genes. (C) 2012 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
Avainsanat:
Antibiotic resistance mechanisms, Bacteroides fragilis, cfiA, Insertion sequence (IS) elements, nim
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