A1 Refereed original research article in a scientific journal
Effects of Antioxidants on Rapeseed Oil Oxidation in an Artificial Digestion Model Analyzed by UHPLC-ESI-MS
Authors: Tarvainen Marko, Phuphusit Aleksandra, Suomela Jukka-Pekka, Kuksis Arnis, Kallio Heikki
Publisher: American Chemical Society
Publication year: 2012
Journal: Journal of Agricultural and Food Chemistry
Journal acronym: J Agric Food Chem
Number in series: 14
Volume: 60
Issue: 14
First page : 3564
Last page: 3579
Number of pages: 16
ISSN: 0021-8561
DOI: https://doi.org/10.1021/jf2050944
Abstract
A normal diet contains large quantities of oxidized fatty acids, glycerolipids, cholesterol, and their cytotoxic degrdn. products because many foods in the diet are fried, heated, or otherwise processed and consumed often after long periods of storage. There is also evidence that the acid medium of the stomach promotes lipid peroxidn. and that the gastrointestinal tract is a major site of antioxidant action, as demonstrated by various colorimetric methods. The identity and yields of specific products of lipid transformation have seldom been detd. The present study describes the mol. species profiles of all major gastrointestinal lipids formed during digestion of autoxidized rapeseed oil in an artificial digestion model in the presence of L-ascorbic acid, 6-palmitoyl-O-L-ascorbic acid, 3,5-di-tert-butyl-4-hydroxytoluene (BHT), DL-α-tocopherol, and DL-α-tocopheryl acetate. Differences in oxidized lipid profiles were detected in the samples digested in the presence of different antioxidants, but none of them could prevent the formation of oxidized lipids or promote their degrdn. in a gastric digestion model. The lack of effect is attributed to the inappropriate nature of the gastrointestinal medium for the antioxidant activity of these vitamins and BHT. A fast ultra high performance liq. chromatog.-electrospray ionization-mass spectrometric method was developed for the anal. of lipolysis products, including epoxy, hydroperoxy, and hydroxy fatty acids, and acylglycerols, utilizing lithium as ionization enhancer.
A normal diet contains large quantities of oxidized fatty acids, glycerolipids, cholesterol, and their cytotoxic degrdn. products because many foods in the diet are fried, heated, or otherwise processed and consumed often after long periods of storage. There is also evidence that the acid medium of the stomach promotes lipid peroxidn. and that the gastrointestinal tract is a major site of antioxidant action, as demonstrated by various colorimetric methods. The identity and yields of specific products of lipid transformation have seldom been detd. The present study describes the mol. species profiles of all major gastrointestinal lipids formed during digestion of autoxidized rapeseed oil in an artificial digestion model in the presence of L-ascorbic acid, 6-palmitoyl-O-L-ascorbic acid, 3,5-di-tert-butyl-4-hydroxytoluene (BHT), DL-α-tocopherol, and DL-α-tocopheryl acetate. Differences in oxidized lipid profiles were detected in the samples digested in the presence of different antioxidants, but none of them could prevent the formation of oxidized lipids or promote their degrdn. in a gastric digestion model. The lack of effect is attributed to the inappropriate nature of the gastrointestinal medium for the antioxidant activity of these vitamins and BHT. A fast ultra high performance liq. chromatog.-electrospray ionization-mass spectrometric method was developed for the anal. of lipolysis products, including epoxy, hydroperoxy, and hydroxy fatty acids, and acylglycerols, utilizing lithium as ionization enhancer.
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