A1 Vertaisarvioitu alkuperäisartikkeli tieteellisessä lehdessä

Bacterial DNA signatures in carotid atherosclerosis represent both commensals and pathogens of skin origin




TekijätRenko J, Koskela KA, Lepp PW, Oksala N, Levula M, Lehtimaki T, Solakivi T, Kunnas T, Nikkari S, Nikkari ST

KustantajaJOHN LIBBEY EUROTEXT LTD

KustannuspaikkaMONTROUGE; 127 AVE DE LA REPUBLIQUE, 92120 MONTROUGE, FRANCE

Julkaisuvuosi2013

JournalEuropean Journal of Dermatology

Tietokannassa oleva lehden nimiEuropean Journal of Dermatology

Lehden akronyymiEur.J.Dermatol.

Numero sarjassa1

Vuosikerta23

Numero1

Aloitussivu53

Lopetussivu58

Sivujen määrä6

ISSN1167-1122

DOIhttps://doi.org/10.1684/ejd.2012.1908


Tiivistelmä
Infectious agents have been suggested to be involved in atherosclerosis. By using a novel subtraction broad-range PCR approach, we defined bacterial DNA signatures in surgically removed sterile carotid artery endarterectomy plaques of patients with carotid atherosclerosis. Eighty partial bacterial 16S rDNA nucleotide sequences from eight patients were studied. Furthermore, 34 clones representing 21 bacterial sequence-types from the reagents used for DNA extraction and PCR amplification were determined. After subtraction of these potential methodological contaminants, 23 bacterial sequence-types were considered as clinically relevant findings. The most prominent phylum, Actinobacteria, accounted for 74 % of these relevant sequences. Furthermore, according to the Human Microbiome project database, interestingly, nearly all (94%) of the sequences were associated with the human skin microbiome.



Last updated on 2024-26-11 at 23:40