Elisa Maria Werner
M.Sc. (TUM) Biochemistry, PhD student

Molecular Plant Biology, Photosynthetic Microbes

elisamaria.werner@utu.fi



Office: 6108


Photosynthetic Microbes




Areas of expertise
Cyanobacteria, alternative electron transfer pathways

Biography

I conducted my Bachelor's and Master's studies in Biochemistry, with minor degrees in industrial biotechnology and microbiology, at the Technical University of Munich. During that time I gathered experience in marine sciences at the Alfred-Wegener-Institute for Polar and Marine Research (Bremerhaven, Germany) and at Hokkaido University (Hakodate, Japan).

After my Master's Thesis at the Werner Siemens-Chair for Synthetic Biotechnology (TUM), I joined the laboratory of Molecular Plant Biology under supervision of Prof. Yagut Allahverdiyeva-Rinne in autum 2021 as a PhD student.



Research

The N2-fixing cyanobacterium Anabaena sp. PCC7120 is a promising bioproduction platform for concomitant reductive biotransformation for value chemical generation in vegetative cells and production of hydrogen in heterocysts. A promising starting point is the deletion of Flavodiiron 3A in vegetative cells, which is part of the auxiliary electron transport chain and subtracts electrons from reductive biotransformations. This deletion also leads to increased H2 production in the heterocysts. The underlying mechanisms for this as well as the roles of Flv2 and Flv4 are to be evaluated. Moreover, a heterologous oxidoreductase is to be introduced for value chemical production. To increase product yield and facilitate downstream processing, the mutant strain is to be immobilized. Investigation of photosynthesis, metabolism and regulatory networks of the mutant strains is  performed using -omics, single reaction monitoring, BiFC combined with FRET, Membrane inlet mass spectrometry (MIMS) and modelling approaches.




Last updated on 2021-28-10 at 00:51